Self-incompatibility alleles in Polish wild pear (Pyrus pyraster (L.) Burgsd.): a preliminary analysis

Wild pear ( Pyrus pyraster , syn. P. communis var. pyraster ) is thought to be one of the species that gave rise to all other members of the genus Pyrus , although intraspecific hybridizations with cultivated varieties could cause the disappearance of original species characteristics. S-RNase allele...

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Bibliographic Details
Published in:Journal of applied genetics 2010-01, Vol.51 (1), p.33-35
Main Authors: Wolko, Ł., Antkowiak, W., Sips, M., Słomski, R.
Format: Article
Language:English
Subjects:
Alleles
Animal Genetics and Genomics
Biomedical and Life Sciences
DNA, Plant
Genes, Plant
Glycoproteins - genetics
Human Genetics
Life Sciences
Microbial Genetics and Genomics
Plant Genetics and Genomics
Polymerase Chain Reaction
Pyrus
Pyrus - enzymology
Pyrus - genetics
Ribonucleases - genetics
Short Communication
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Summary:Wild pear ( Pyrus pyraster , syn. P. communis var. pyraster ) is thought to be one of the species that gave rise to all other members of the genus Pyrus , although intraspecific hybridizations with cultivated varieties could cause the disappearance of original species characteristics. S-RNase alleles from 7 different wild pear individuals, collected from various regions of Poland, were cloned on the basis of the PCR method and nucleotide sequence analyses. The hypervariable (HV) region is responsible for allele-specific S-RNase activity in the self-incompatibility mechanism. The high level of polymorphism of its sequences may constitute a source of valuable phylogenetic information. From all individuals, 14 sequences were obtained successfully, and 9 of them were novel alleles. Phylogenetic analysis of these alleles was based on the amino acid sequence interpretation of coding regions and intron nucleotide sequences. The research conducted on a limited pool of available P. pyraster alleles gives only an initial insight into possible S-RNase allele polymorphisms in wild populations. At this stage, the results do not confirm a strong influence of cultivated pear species on the wild pear.
ISSN:1234-1983
2190-3883
DOI:10.1007/BF03195708