Planktonic and Biofilm Communities from 7-Day-Old Chicken Cecal Microflora Cultures: Characterization and Resistance to Salmonella Colonization

• Published in: Journal of food protection 2009-09, Vol.72 (9), p.1812-1820
• Main Authors: Sheffield, C.L, Crippen, T.L, Andrews, K, Bongaerts, R.J, Nisbet, D.J
• Format: Article
• Language: English
• Subjects: animal pathogenic bacteria; Animals; Animals, Newborn; Antibiotics; automatic detection; Automation; bacterial adhesion; bacterial colonization; biofilm; Biofilms; Biofilms - growth & development; biological control; biotypes; Biotyping; cecum; Cecum - microbiology; Chickens; chicks; Colonization; colonizing ability; Colony Count, Microbial; competitive exclusion; Consumer Product Safety; culture media; Disease Susceptibility - veterinary; Food Contamination - prevention & control; Food safety; Gastrointestinal tract; Humans; intestinal microorganisms; isolation; Klebsiella pneumoniae; lactic acid bacteria; Microbiology; Microorganisms; plankton; Poultry; Poultry Diseases - prevention & control; Probiotics; ribotypes; Ribotyping; Salmonella; Salmonella enterica; Salmonella enterica subsp. enterica serovar Typhimurium; Salmonella Infections, Animal - prevention & control; Salmonella typhimurium - growth & development; Salmonella typhimurium - physiology
• ISSN: 0362-028X; 1944-9097
• DOI: 10.4315/0362-028X-72.9.1812

Information implicating bacterial biofilms as contributory factors in the development of environmental bacterial resistance has been increasing. There is a lack of information regarding the role of biofilms within the microbial ecology of the gastrointestinal tract of food animals. This work used a continuous-flow chemostat model derived from the ceca of 7-day-old chicks to characterize these communities and their ability to neutralize invasion by Salmonella enterica serovar Typhimurium. We characterized and compared the biofilm and planktonic communities within these microcosms using automated ribotyping and the Analytical Profile Index biotyping system. Eleven species from eight different genera were identified from six culture systems. Klebsiella pneumoniae was isolated from all planktonic communities and four of the biofilm communities. Three of the communities resisted colonization by Salmonella enterica serovar Typhimurium, two communities suppressed growth, and one community succumbed to colonization. In cultures that resisted colonization, no Salmonella could be isolated from the biofilm; in cultures that succumbed to colonization, Salmonella was consistently found within the biofilms. This study was one of a series that provided a molecular-based characterization of both the biofilm and planktonic communities from continuous-flow culture systems derived from the cecal microflora of chicks, ranging in age from day-of-hatch to 14 days old. The one common factor relating to successful colonization of the culture was the presence of Salmonella within the biofilm. The capacity to sequester the introduced Salmonella into the biofilm appears to be a contributing factor to the inability of these cultures to withstand colonization by the Salmonella.