Morphological and biochemical properties of a new human breast cancer cell line

DU4475 is a new human breast cell line derived from a cutaneous metastatic nodule from a patient with advanced breast cancer. It has been in continuous culture for more than 1 year and has survived 140 subcultivations. The cells grow in suspension, displaying clusters and free-floating aggregates wi...

Full description

Saved in:
Bibliographic Details
Published in:Cancer research (Chicago, Ill.) Ill.), 1979-07, Vol.39 (7 Pt 1), p.2604-2613
Main Authors: Langlois, A J, Holder, Jr, W D, Iglehart, J D, Nelson-Rees, W A, Wells, Jr, S A, Bolognesi, D P
Format: Article
Language:English
Subjects:
Breast Neoplasms - genetics
Breast Neoplasms - metabolism
Breast Neoplasms - pathology
Cell Line
Chromosomes
Female
Glucosephosphate Dehydrogenase - analysis
Humans
Isoenzymes - analysis
Neoplasm Transplantation
Receptors, Cell Surface - analysis
Transplantation, Heterologous
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:DU4475 is a new human breast cell line derived from a cutaneous metastatic nodule from a patient with advanced breast cancer. It has been in continuous culture for more than 1 year and has survived 140 subcultivations. The cells grow in suspension, displaying clusters and free-floating aggregates with serpentine-like outgrowths. The cell line is hypotetraploid with modal chromosome numbers between 86 and 93 and possesses five to six distinctive marker chromosomes. The cells grow readily in athymic nude mice and produce tumors from which cells can be reintroduced to culture in vitro.
ISSN:0008-5472