NMR resonance assignments of an engineered neomycin-sensing riboswitch RNA bound to ribostamycin and tobramycin

• Published in: Biomolecular NMR assignments 2010-04, Vol.4 (1), p.115-118
• Main Authors: Schmidtke, Sina R, Duchardt-Ferner, Elke, Weigand, Julia E, Suess, Beatrix, Wöhnert, Jens
• Format: Article
• Language: English
• Subjects: aminoglycosides; Binding sites; Biochemistry; Biological and Medical Physics; Biophysics; Carbon Isotopes - chemistry; Gene expression; gene expression regulation; Hydrogen - chemistry; Nitrogen Isotopes - chemistry; NMR; NMR-assignments; Nuclear magnetic resonance; Nuclear Magnetic Resonance, Biomolecular; Phosphorus Isotopes - chemistry; Physics; Physics and Astronomy; Polymer Sciences; Protein Engineering; Regulatory Sequences, Ribonucleic Acid; Ribostamycin - chemistry; Ribostamycin - metabolism; Riboswitch; RNA; RNA, Fungal - chemistry; RNA, Fungal - metabolism; RNA, Messenger - chemistry; RNA, Messenger - metabolism; Saccharomyces cerevisiae; Tobramycin - chemistry; Tobramycin - metabolism; Triple resonance experiments; Yeast
• ISSN: 1874-2718; 1874-270X
• DOI: 10.1007/s12104-010-9223-z

The neomycin-sensing riboswitch is an engineered riboswitch developed to regulate gene expression in vivo in the lower eukaryote Saccharomyces cerevisiae upon binding to neomycin B. With a size of only 27nt it is the smallest functional riboswitch element identified so far. It binds not only neomycin B but also related aminoglycosides of the 2′-deoxystreptamine class with high affinity. The regulatory activity, however, strongly depends on the identity of the aminoglycoside. As a prerequisite for the structure determination of riboswitch-ligand complexes we report here the ¹H, ¹⁵N, ¹³C and partial ³¹P chemical shift assignments for the minimal functional 27nt neomycin sensing riboswitch RNA in complex with the 4,5-linked neomycin analog ribostamycin and the 4,6-linked aminoglycoside tobramycin.