Simultaneous expression of choline oxidase, superoxide dismutase and ascorbate peroxidase in potato plant chloroplasts provides synergistically enhanced protection against various abiotic stresses

Plants synthesize compatible solutes such as glycinebetaine (GB) in response to abiotic stresses. To evaluate the synergistic and protective effect of GB, transgenic potato plants expressing superoxide dismutase (SOD) and ascorbate peroxidase (APX) targeting to chloroplasts (referred to as SSA plant...

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Published in:Physiologia plantarum 2010-04, Vol.138 (4), p.520-533
Main Authors: Ahmad, Raza, Kim, Yun-Hee, Kim, Myoung-Duck, Kwon, Suk-Yoon, Cho, Kwangsoo, Lee, Haeng-Soon, Kwak, Sang-Soo
Format: Article
Language:English
Subjects:
Adaptation, Physiological - drug effects
Alcohol Oxidoreductases - genetics
Alcohol Oxidoreductases - metabolism
Ascorbate Peroxidases
Betaine - metabolism
Blotting, Western
Chloroplasts - enzymology
Chloroplasts - genetics
Droughts
Gene Expression Regulation, Enzymologic
Gene Expression Regulation, Plant
Oxidative Stress
Paraquat - pharmacology
Peroxidases - genetics
Peroxidases - metabolism
Plants, Genetically Modified
Reverse Transcriptase Polymerase Chain Reaction
Sodium Chloride - pharmacology
Solanum tuberosum
Solanum tuberosum - enzymology
Solanum tuberosum - genetics
Superoxide Dismutase - genetics
Superoxide Dismutase - metabolism
Water - pharmacology
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Summary:Plants synthesize compatible solutes such as glycinebetaine (GB) in response to abiotic stresses. To evaluate the synergistic and protective effect of GB, transgenic potato plants expressing superoxide dismutase (SOD) and ascorbate peroxidase (APX) targeting to chloroplasts (referred to as SSA plants) were retransformed with a bacterial choline oxidase (codA) gene to synthesize GB in chloroplast in naturally occurring non-accumulator potato plants (including SSA) under the control of the stress-inducible SWPA2 promoter (referred to as SSAC plants). GB accumulation resulted in enhanced protection of these SSAC plants and lower levels of H₂O₂ compared with SSA and non-transgenic (NT) plants after methyl viologen (MV)-mediated oxidative stress. Additionally, SSAC plants demonstrated synergistically enhanced tolerance to salt and drought stresses at the whole-plant level. GB accumulation in SSAC plants helped to maintain higher activities of SOD, APX and catalase following oxidative, salt and drought stress treatments than is observed in SSA and NT plants. Conclusively, GB accumulation in SSAC plants along with overexpression of antioxidant genes rendered the plants tolerant to multiple environmental stresses in a synergistic fashion.
ISSN:0031-9317
1399-3054
DOI:10.1111/j.1399-3054.2010.01348.x