Rapid quantification of itraconazole-resistant Aspergillus fumigatus in air

Solid-phase cytometry (SPC) was used to determine the total number and the number of itraconazole-resistant Aspergillus fumigatus cells in 60 air samples. Of the 570 A. fumigatus cells that were recovered, 10 (1.8%) were resistant. SPC proved more specific and rapid than culture and allowed high-tro...

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Bibliographic Details
Published in:Journal of microbiological methods 2010-05, Vol.81 (2), p.197-199
Main Authors: Vanhee, Lies M.E., Perman, Dana, Nelis, Hans J., Coenye, Tom
Format: Article
Language:English
Subjects:
Air Microbiology
Air sampling
Antifungal Agents - pharmacology
Aspergillus
Aspergillus fumigatus
Aspergillus fumigatus - drug effects
Aspergillus fumigatus - isolation & purification
Biological and medical sciences
Cell culture
Colony Count, Microbial - methods
Drug Resistance, Fungal
Fundamental and applied biological sciences. Psychology
Humans
Itraconazole - pharmacology
Itraconazole resistance
Microbiology
Mycological methods and techniques used in mycology
Mycology
Mycology - methods
Solid-phase cytometry
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Summary:Solid-phase cytometry (SPC) was used to determine the total number and the number of itraconazole-resistant Aspergillus fumigatus cells in 60 air samples. Of the 570 A. fumigatus cells that were recovered, 10 (1.8%) were resistant. SPC proved more specific and rapid than culture and allowed high-troughput susceptibility testing.
ISSN:0167-7012
1872-8359
DOI:10.1016/j.mimet.2010.02.012