Bovine papillomavirus type 1 E2 and E7 proteins down-regulate Toll Like Receptor 4 ( TLR4) expression in equine fibroblasts

BPV-1 and less commonly BPV-2 are associated with the pathogenesis of equine skin tumours termed sarcoids. We recently documented the transcriptional changes that are induced by BPV-1 in equine fibroblasts using microarray analyses. TLR4 expression was found to be significantly down-regulated by BPV...

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Bibliographic Details
Published in:Virus research 2010-04, Vol.149 (1), p.124-127
Main Authors: Yuan, Z.Q., Bennett, L., Campo, M.S., Nasir, L.
Format: Article
Language:English
Subjects:
Animals
Bovine papillomavirus
Bovine papillomavirus 1 - immunology
Bovine papillomavirus 1 - pathogenicity
Cells, Cultured
DNA-Binding Proteins - antagonists & inhibitors
DNA-Binding Proteins - immunology
Down-Regulation
Equidae
Fibroblasts - virology
Gene Silencing
Host-Pathogen Interactions
Oncogene Proteins, Viral - antagonists & inhibitors
Oncogene Proteins, Viral - immunology
RNA, Small Interfering - genetics
Toll Like Receptor 4
Toll-Like Receptor 4 - biosynthesis
Viral Proteins - antagonists & inhibitors
Viral Proteins - immunology
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Summary:BPV-1 and less commonly BPV-2 are associated with the pathogenesis of equine skin tumours termed sarcoids. We recently documented the transcriptional changes that are induced by BPV-1 in equine fibroblasts using microarray analyses. TLR4 expression was found to be significantly down-regulated by BPV-1. In the present study, we show that TLR4 expression is significantly decreased following the exogenous expression of BPV-1 E2 and E7 in primary equine fibroblasts. The results were confirmed by the demonstration of increased TLR4 expression following siRNA suppression of BPV-1 E2 and E7 viral gene expression. These data imply that BPV-1 is able to subvert the innate immune response by downregulation of TLR4.
ISSN:0168-1702
1872-7492
DOI:10.1016/j.virusres.2010.01.008