Determination and toxicokinetics comparison of ketamine and S(+)-ketamine in dog plasma by HPLC-MS method

;A simple and reproducible method was developed for the quantification of ketamine and S(+)‐ketamine in dog plasma using a high‐performance liquid chromatography system coupled to a positive ion electrospray mass spectrometric analysis. Solid‐phase extraction was used for extracting analytes from do...

Full description

Saved in:
Bibliographic Details
Published in:Biomedical chromatography 2010-03, Vol.24 (3), p.253-260
Main Authors: Sheng, Yu-xin, Zhang, Ying-hao, Zhang, Jin-lan, Li, Jin, Li, Hui, Jin, Hong-tao
Format: Article
Language:English
Subjects:
Animals
Chromatography, High Pressure Liquid - economics
Chromatography, High Pressure Liquid - methods
Dogs
Female
HPLC-MS
ketamine
Ketamine - administration & dosage
Ketamine - blood
Male
S (+)-ketamine
Sensitivity and Specificity
Solid Phase Extraction - methods
Spectrometry, Mass, Electrospray Ionization - economics
Spectrometry, Mass, Electrospray Ionization - methods
Time Factors
toxicokinetics
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:;A simple and reproducible method was developed for the quantification of ketamine and S(+)‐ketamine in dog plasma using a high‐performance liquid chromatography system coupled to a positive ion electrospray mass spectrometric analysis. Solid‐phase extraction was used for extracting analytes from dog plasma samples. The analytes were separated on a Zorbax SB C18 column (100 × 2.1 mm, 3.5 μm) with acetonitrile–formate buffer (10 mM ammonium formate and 0.3% formic acid) (17 : 83, v/v) as mobile phase at a flow‐rate of 0.2 mL/min. Detection was operated under selected ion monitoring mode. [M + H]+ at m/z 238 for ketamine and S(+)‐ketamine and [M + H]+ at m/z 180 for phenacetin (internal standard) were selected as detecting ions, respectively. The method was linear in the concentration range 51.6–2580 ng/mL. The intra‐ and inter‐day precisions (RSD %) were within 11.3% and the assay accuracies ranged from 80.0 to 101.4%. Their average recoveries were greater than 91.1% at all test concentrations. The analytes were proved to be stable during all sample storage, preparation and analysis procedures. The method was successfully applied to the toxicokinetics study and comparison of ketamine and S (+)‐ketamine following intravenous administration to dogs. Copyright © 2009 John Wiley & Sons, Ltd.
ISSN:0269-3879
1099-0801
DOI:10.1002/bmc.1280