Iron-mediated suppression of bloom-forming cyanobacteria by oxine in a eutrophic lake

• Published in: Freshwater biology 2010-05, Vol.55 (5), p.1102-1117
• Main Authors: MOLOT, LEWIS A, LI, GUIYOU, FINDLAY, DAVID L, WATSON, SUSAN B
• Format: Article
• Language: English
• Subjects: Anabaena flos-aquae; Aphanizomenon; Cyanobacteria; cyanobacteria bloom; eutrophic; Freshwater; iron; oxine; Pseudokirchneriella subcapitata; Scenedesmus quadricauda; Synechococcus
• ISSN: 0046-5070; 1365-2427
• DOI: 10.1111/j.1365-2427.2009.02384.x

1. Published studies show that cyanobacteria have higher Fe requirements than eukaryotic algae. To test whether Fe availability can affect formation of a cyanobacterial bloom, a strong Fe chelator, oxine (8-hydroxyquinoline, C₉H₇NO), was added to enclosures in eutrophic Lake 227 in the Experimental Lakes Area (ELA) (northwestern Ontario). 2. Aphanizomenon schindlerii growth was suppressed, and growth of eukaryotic chlorophytes significantly promoted in enclosures to which oxine had been added. Significant eukaryotic growth did not occur in enclosures treated with ammonium, suggesting that N supplied by degradation of oxine was not responsible for eukaryotic success in the oxine enclosures. 3. In situ Fe²⁺ measurements were unreliable because of interference from high concentrations of dissolved organic compounds. However, oxine rapidly promoted oxidation of Fe²⁺ to Fe³⁺ in deionised water, suggesting that rapid removal of Fe²⁺ also occurred in the oxine-treated enclosures. 4. In batch cultures, 10 μ m Fe and 10 μ m oxine (a 1 : 1 ratio) completely inhibited the growth of the cyanobacteria Synechococcus sp. and Anabaena flos-aquae and the chlorophytes Pseudokirchneriella subcapitata and Scenedesmus quadricauda. Increasing Fe 10-fold to 100 μ m Fe completely and partially reversed oxine inhibition in the two chlorophytes but could not overcome inhibition of the cyanobacteria, indicating that inhibition was Fe-mediated at least in the eukaryotes. Since oxine binds Fe³⁺ in a 1 : 3 ratio (Fe : oxine), inhibition at a 1 : 1 ratio indicates that not all of the Fe is bound, and a mechanism involving Fe other than chelation was at least partly responsible for inhibition. 5. Collectively, the enclosure and laboratory results suggest that the outcome of competition between cyanobacteria and eukaryotic algae in the oxine-treated enclosures in Lake 227 was likely a result of decreased availability of Fe, especially Fe²⁺. 6. The results suggest that remediation methods that dramatically restrict the supply rate of Fe²⁺ could reduce the relative abundance of cyanobacteria in eutrophic systems.