A computerized analysis of the entire retinal ganglion cell population and its spatial distribution in adult rats

In adult albino (SD) and pigmented (PVG) rats the entire population of retinal ganglion cells (RGCs) was quantified and their spatial distribution analyzed using a computerized technique. RGCs were back-labelled from the optic nerves (ON) or the superior colliculi (SCi) with Fluorogold (FG). Numbers...

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Bibliographic Details
Published in:Vision research (Oxford) 2009, Vol.49 (1), p.115-126
Main Authors: Salinas-Navarro, M., Mayor-Torroglosa, S., Jiménez-López, M., Avilés-Trigueros, M., Holmes, T.M., Lund, R.D., Villegas-Pérez, M.P., Vidal-Sanz, M.
Format: Article
Language:English
Subjects:
Animals
Automatic Data Processing
Biological and medical sciences
Cell Count
Computerized image analysis
Eye and associated structures. Visual pathways and centers. Vision
Female
Fluorescent Dyes
Fluorescent tracers
Fundamental and applied biological sciences. Psychology
Male
Microscopy, Fluorescence
Optic Nerve
Rat
Rats
Rats, Inbred BN
Rats, Sprague-Dawley
Reproducibility of Results
Retina
Retinal ganglion cells
Retinal Ganglion Cells - cytology
Retrograde labelling
Species Specificity
Superior Colliculi
Vertebrates: nervous system and sense organs
Visual streak
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Summary:In adult albino (SD) and pigmented (PVG) rats the entire population of retinal ganglion cells (RGCs) was quantified and their spatial distribution analyzed using a computerized technique. RGCs were back-labelled from the optic nerves (ON) or the superior colliculi (SCi) with Fluorogold (FG). Numbers of RGCs labelled from the ON [SD: 82,818 ± 3,949, n = 27; PVG: 89,241 ± 3,576, n = 6) were comparable to those labelled from the SCi [SD: 81,486 ± 4,340, n = 37; PVG: 87,229 ± 3,199; n = 59]. Detailed methodology to provide cell density information at small scales demonstrated the presence of a horizontal region in the dorsal retina with highest densities, resembling a visual streak.
ISSN:0042-6989
1878-5646
DOI:10.1016/j.visres.2008.09.029