Improving the expression of mini-proinsulin in Pichia pastoris

Increased expression of recombinant mini-proinsulin in Pichia pastoris in 2.5 l bioreactors was achieved by increasing the cultivation pH from 5.1 to 6.3, by decreasing the temperature from 28 to 22 °C, and by periodical addition of ammonium sulfate and EDTA to the culture broth. Using this procedur...

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Bibliographic Details
Published in:Biotechnology letters 2004-08, Vol.26 (16), p.1269-1272
Main Authors: Pais-Chanfrau, J.M, Garcia, Y, Licor, L, Besada, V, Castellanos-Serra, L, Cabello, C.I, Hernandez, L, Mansur, M, Plana, L, Hidalgo, A
Format: Article
Language:English
Subjects:
Ammonium
Ammonium Sulfate - pharmacology
Biological and medical sciences
Bioreactors
Bioreactors - microbiology
Biotechnology
Cell Culture Techniques - methods
Cell Proliferation - drug effects
Edetic Acid - pharmacology
Fundamental and applied biological sciences. Psychology
gene expression
humans
Hydrogen-Ion Concentration
insulin
Microbiology
Nitrogen - metabolism
pH effects
Pichia - drug effects
Pichia - genetics
Pichia - growth & development
Pichia - metabolism
Pichia pastoris
proinsulin
Proinsulin - biosynthesis
Proinsulin - genetics
protein expression systems
Q1
Q2
recombinant proteins
Recombinant Proteins - biosynthesis
Temperature
Temperature effects
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Summary:Increased expression of recombinant mini-proinsulin in Pichia pastoris in 2.5 l bioreactors was achieved by increasing the cultivation pH from 5.1 to 6.3, by decreasing the temperature from 28 to 22 °C, and by periodical addition of ammonium sulfate and EDTA to the culture broth. Using this procedure, mini-proinsulin reached nearly 0.3 g l-1 in the culture supernatant after 160 h of growth.
ISSN:0141-5492
1573-6776
DOI:10.1023/B:BILE.0000044871.80632.f9