A multicenter trial to compare blood culture with polymerase chain reaction in severe human sepsis

Objective To assess the presence of microbial DNA in the blood by polymerase chain reaction (PCR) and its association with disease severity and markers of inflammation in severe sepsis and to compare the performance of PCR with blood culture (BC). Design Prospective multicentric controlled observati...

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Bibliographic Details
Published in:Intensive care medicine 2010-02, Vol.36 (2), p.241-247
Main Authors: Bloos, Frank, Hinder, Frank, Becker, Karsten, Sachse, Svea, Mekontso Dessap, Armand, Straube, Eberhard, Cattoir, Vincent, Brun-Buisson, Christian, Reinhart, Konrad, Peters, Georg, Bauer, Michael
Format: Article
Language:English
Subjects:
Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy
Anesthesiology
Biological and medical sciences
Blood
Blood. Blood and plasma substitutes. Blood products. Blood cells. Blood typing. Plasmapheresis. Apheresis
Canada
Critical Care Medicine
DNA, Viral - genetics
Emergency Medicine
Ethylenediaminetetraacetic acid
Female
Germany
Hospitals, Teaching - statistics & numerical data
Humans
Infection
Intensive
Intensive care medicine
Intensive Care Units - statistics & numerical data
Male
Medical examination
Medical sciences
Medicine
Medicine & Public Health
Middle Aged
Mortality
Original
Pain Medicine
Pediatrics
Pneumology/Respiratory System
Polymerase chain reaction
Polymerase Chain Reaction - methods
Prospective Studies
Sepsis - blood
Sepsis - genetics
Sepsis - microbiology
Severity of Illness Index
Transfusions. Complications. Transfusion reactions. Cell and gene therapy
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Summary:Objective To assess the presence of microbial DNA in the blood by polymerase chain reaction (PCR) and its association with disease severity and markers of inflammation in severe sepsis and to compare the performance of PCR with blood culture (BC). Design Prospective multicentric controlled observational study. Setting Three surgical intensive care units in university centers and large teaching hospitals. Patients One hundred forty-two patients with severe sepsis and 63 surgical controls. Interventions Presence of microbial DNA was assessed by multiplex PCR upon enrollment, and each time a BC was obtained. Measurements and main results Controls had both approximately 4% positive PCRs and BCs. In severe sepsis, 34.7% of PCRs were positive compared to 16.5% of BCs ( P  
ISSN:0342-4642
1432-1238
DOI:10.1007/s00134-009-1705-z