Antibacterial and biofilm removal activity of a podoviridae Staphylococcus aureus bacteriophage SAP-2 and a derived recombinant cell-wall-degrading enzyme

• Published in: Applied microbiology and biotechnology 2010-05, Vol.86 (5), p.1439-1449
• Main Authors: Son, Jee-Soo, Lee, Se-Jung, Jun, Soo Youn, Yoon, Seong Jun, Kang, Sang Hyeon, Paik, Hyoung Rok, Kang, Jung Ok, Choi, Yun-Jaie
• Format: Article
• Language: English
• Subjects: Agricultural biotechnology; Amino Acid Sequence; Animals; Anti-Bacterial Agents - pharmacology; Antibiotics; Bacteria; Bacterial infections; Bacteriolysis; Biofilms; Biological and medical sciences; Biomedical and Life Sciences; Biotechnologically Relevant Enzymes and Proteins; Biotechnology; Cattle; Cell Wall - metabolism; Cells; E coli; Endopeptidases - genetics; Endopeptidases - isolation & purification; Endopeptidases - metabolism; Enzymes; Escherichia coli; Fundamental and applied biological sciences. Psychology; Genome, Viral; Genomes; Life Sciences; Low temperature; Microbial Genetics and Genomics; Microbial Sensitivity Tests; Microbiology; Molecular Sequence Data; Morphology; Penicillin; Podoviridae; Podoviridae - enzymology; Podoviridae - isolation & purification; Podoviridae - physiology; Podoviridae - ultrastructure; Recombinant Proteins - metabolism; Staphylococcal Infections - microbiology; Staphylococcal Infections - therapy; Staphylococcal Infections - virology; Staphylococcus aureus; Staphylococcus aureus - metabolism; Staphylococcus aureus - virology; Staphylococcus infections; Staphylococcus Phages - enzymology; Staphylococcus Phages - isolation & purification; Staphylococcus Phages - physiology; Staphylococcus Phages - ultrastructure; Studies
• ISSN: 0175-7598; 1432-0614
• DOI: 10.1007/s00253-009-2386-9

Antibacterial and biofilm removal activity of a new podoviridae Staphylococcus aureus bacteriophage (SAP-2), which belongs to the φ29-like phage genus of the Podoviridae family, and a cell-wall-degrading enzyme (SAL-2), which is derived from bacteriophage SAP-2, have been characterized. The cell-wall-degrading enzyme SAL-2 was expressed in Escherichia coli in a soluble form using a low-temperature culture. The cell-wall-degrading enzyme SAL-2 had specific lytic activity against S. aureus, including methicillin-resistant strains, and showed a minimum inhibitory concentration of about 1 μg/ml. In addition, this enzyme showed a broader spectrum of activity within the Staphylococcus genus compared with bacteriophage SAP-2 in its ability to remove the S. aureus biofilms. Thus, the cell-wall-degrading enzyme SAL-2 can be used to prevent and treat biofilm-associated S. aureus infections either on its own or in combination with other cell-wall-degrading enzymes with anti-S. aureus activity.