Single-cell analysis and isolation for microbiology and biotechnology: methods and applications

Various single-cell isolation techniques, including dilution, micromanipulation, flow cytometry, microfluidics, and compartmentalization, have been developed. These techniques can be used to cultivate previously uncultured microbes, to assess and monitor cell physiology and function, and to screen f...

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Bibliographic Details
Published in:Applied microbiology and biotechnology 2010-05, Vol.86 (5), p.1281-1292
Main Authors: Ishii, Satoshi, Tago, Kanako, Senoo, Keishi
Format: Article
Language:English
Subjects:
Animals
Bacteria - isolation & purification
Biological and medical sciences
Biomedical and Life Sciences
Biotechnology
Biotechnology - methods
Cells
Cloning
Extinction
Flow cytometry
Fluid dynamics
Fluorescence
Fundamental and applied biological sciences. Psychology
Fungi - isolation & purification
Humans
Lasers
Life Sciences
Microbial Genetics and Genomics
Microbiological Techniques
Microbiology
Microorganisms
Mini-Review
Physiology
Studies
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Description
Summary:Various single-cell isolation techniques, including dilution, micromanipulation, flow cytometry, microfluidics, and compartmentalization, have been developed. These techniques can be used to cultivate previously uncultured microbes, to assess and monitor cell physiology and function, and to screen for novel microbiological products. Various other techniques, such as viable staining, in situ hybridization, and those using autofluorescence proteins, are frequently combined with these single-cell isolation techniques depending on the purpose of the study. In this review article, we summarize currently available single-cell isolation techniques and their applications, when used in combination with other techniques, in microbiological and biotechnological studies.
ISSN:0175-7598
1432-0614
DOI:10.1007/s00253-010-2524-4