Single-cell analysis and isolation for microbiology and biotechnology: methods and applications

Various single-cell isolation techniques, including dilution, micromanipulation, flow cytometry, microfluidics, and compartmentalization, have been developed. These techniques can be used to cultivate previously uncultured microbes, to assess and monitor cell physiology and function, and to screen f...

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Published in:Applied microbiology and biotechnology 2010-05, Vol.86 (5), p.1281-1292
Main Authors: Ishii, Satoshi, Tago, Kanako, Senoo, Keishi
Format: Article
Language:English
Subjects:
Animals
Bacteria - isolation & purification
Biological and medical sciences
Biomedical and Life Sciences
Biotechnology
Biotechnology - methods
Cells
Cloning
Extinction
Flow cytometry
Fluid dynamics
Fluorescence
Fundamental and applied biological sciences. Psychology
Fungi - isolation & purification
Humans
Lasers
Life Sciences
Microbial Genetics and Genomics
Microbiological Techniques
Microbiology
Microorganisms
Mini-Review
Physiology
Studies
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cites cdi_FETCH-LOGICAL-c522t-fe8fca41a0724b0b7b118b60ae121e2aee4677ba0d4dc3416472ef1bcadf79353
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container_title Applied microbiology and biotechnology
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creator Ishii, Satoshi
Tago, Kanako
Senoo, Keishi
description Various single-cell isolation techniques, including dilution, micromanipulation, flow cytometry, microfluidics, and compartmentalization, have been developed. These techniques can be used to cultivate previously uncultured microbes, to assess and monitor cell physiology and function, and to screen for novel microbiological products. Various other techniques, such as viable staining, in situ hybridization, and those using autofluorescence proteins, are frequently combined with these single-cell isolation techniques depending on the purpose of the study. In this review article, we summarize currently available single-cell isolation techniques and their applications, when used in combination with other techniques, in microbiological and biotechnological studies.
doi_str_mv 10.1007/s00253-010-2524-4
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source ABI/INFORM Global; Springer Nature
subjects Animals
Bacteria - isolation & purification
Biological and medical sciences
Biomedical and Life Sciences
Biotechnology
Biotechnology - methods
Cells
Cloning
Extinction
Flow cytometry
Fluid dynamics
Fluorescence
Fundamental and applied biological sciences. Psychology
Fungi - isolation & purification
Humans
Lasers
Life Sciences
Microbial Genetics and Genomics
Microbiological Techniques
Microbiology
Microorganisms
Mini-Review
Physiology
Studies
title Single-cell analysis and isolation for microbiology and biotechnology: methods and applications
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