Proteome of Gluconacetobacter diazotrophicus co-cultivated with sugarcane plantlets

Gluconacetobacter diazotrophicus is a micro-aerobic bacterium able to fix atmospheric nitrogen in endophytic mode. A proteomic approach was used to analyze proteins differentially expressed in the presence and absence of sugarcane plantlets. Two-dimensional gel electrophoresis (2-DE) showed 42 spots...

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Bibliographic Details
Published in:Journal of proteomics 2010-03, Vol.73 (5), p.917-931
Main Authors: dos Santos, Marise Fonseca, Muniz de Pádua, Vânia Lúcia, de Matos Nogueira, Eduardo, Hemerly, Adriana Silva, Domont, Gilberto Barbosa
Format: Article
Language:English
Subjects:
Biological and medical sciences
Carbohydrates
Chaperonins
Differential protein expression
Diverse techniques
Endophytes
Endophytic interaction
Energy metabolism
Fundamental and applied biological sciences. Psychology
Gel electrophoresis
Gluconacetobacter diazotrophicus
Ionization
Lasers
Lipoproteins
Molecular and cellular biology
Nitrogen
outer membrane proteins
Outer membranes
Plantlets
Proteome analysis
proteomics
Sugarcane
Symbiosis
Transcription elongation
Translation
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Summary:Gluconacetobacter diazotrophicus is a micro-aerobic bacterium able to fix atmospheric nitrogen in endophytic mode. A proteomic approach was used to analyze proteins differentially expressed in the presence and absence of sugarcane plantlets. Two-dimensional gel electrophoresis (2-DE) showed 42 spots with altered levels of expression. Analysis of these spots by matrix-assisted laser desorption ionization time-of-flight in tandem (MALDI-TOF-TOF) identified 38 proteins. Differentially expressed proteins were associated with carbohydrate and energy metabolism, folding, sorting and degradation processes, and transcription and translation. Among proteins expressed in co-cultivated bacteria, four belong to membrane systems; others, like a transcription elongation factor (GreA), a 60 kDa chaperonin (GroEL), and an outer membrane lipoprotein (Omp16) have also been described in other plant–bacteria associations, indicating a common protein expression pattern as a result of symbiosis. A high protein content of 60 kDa chaperonin isoforms was detected as non-differentially expressed proteins of the bacteria proteome. These results allow the assessment of the physiological significance of specific proteins to G. diazotrophicus metabolism and to the pathways involved in bacteria –host endophytic interaction.
ISSN:1874-3919
DOI:10.1016/j.jprot.2009.12.005