Effect of the association of reduced glutathione and ciprofloxacin on the antimicrobial activity in Staphylococcus aureus

We report the effect of glutathione and the role of reactive oxygen species (ROS), assayed by a nitro blue tetrazolium reaction, on the antibacterial action of ciprofloxacin, gentamicin and chloramphenicol in Staphylococcus aureus 22 resistant to ciprofloxacin and gentamicin, and in S. aureus ATCC 2...

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Bibliographic Details
Published in:FEMS microbiology letters 2010-02, Vol.303 (1), p.101-105
Main Authors: Páez, Paulina Laura, Becerra, María Cecilia, Albesa, Inés
Format: Article
Language:English
Subjects:
Anti-Bacterial Agents - pharmacology
Antibiotics
Antiinfectives and antibacterials
Antimicrobial activity
Bacteriology
Biological and medical sciences
Chloramphenicol
Chloramphenicol - pharmacology
Chloromycetin
Ciprofloxacin
Ciprofloxacin - pharmacology
Drug Interactions
Fundamental and applied biological sciences. Psychology
Gentamicin
Gentamicins - pharmacology
Glutathione
Glutathione - pharmacology
Intracellular
Microbial Sensitivity Tests
Microbiology
Minimum inhibitory concentration
Miscellaneous
Oxidative stress
Pathogens
Reactive oxygen species
Reactive Oxygen Species - metabolism
Staphylococcus aureus
Staphylococcus aureus - drug effects
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Summary:We report the effect of glutathione and the role of reactive oxygen species (ROS), assayed by a nitro blue tetrazolium reaction, on the antibacterial action of ciprofloxacin, gentamicin and chloramphenicol in Staphylococcus aureus 22 resistant to ciprofloxacin and gentamicin, and in S. aureus ATCC 29213 sensitive to the above three antibiotics. The association of glutathione with ciprofloxacin or gentamicin significantly reduced the value of the minimum inhibitory concentration (MIC) in resistant S. aureus 22, measured using the macrodilution method, with a concomitant increase of intracellular ROS and a decrease of extracellular ROS. However, glutathione did not induce modifications in MIC or ROS generated by chloramphenicol. Furthermore, in the sensitive S. aureus ATCC 29213, the association of glutathione with ciprofloxacin, gentamicin or chloramphenicol did not induce any significant variations of MIC or ROS. There was a correlation between the stimulus of intracellular ROS and the decrease of MIC caused by exogenous glutathione. According to the results obtained, it is possible to modify the sensitivity of resistant strains of S. aureus by the addition of exogenous glutathione.
ISSN:0378-1097
1574-6968
DOI:10.1111/j.1574-6968.2009.01867.x