High-level transgene expression in neurons by lentivirus with Tet-Off system

We developed novel lentiviral vectors by using “Tet-Off system” and succeeded in achieving high-level and neuron-specific gene transduction in vivo. One week after viral injection into the rat neostriatum, the GFP expression was almost completely neuron-specific and about 40 times higher than the ex...

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Bibliographic Details
Published in:Neuroscience research 2009-02, Vol.63 (2), p.149-154
Main Authors: Hioki, Hiroyuki, Kuramoto, Eriko, Konno, Michiteru, Kameda, Hiroshi, Takahashi, Yasuhiro, Nakano, Takashi, Nakamura, Kouichi C., Kaneko, Takeshi
Format: Article
Language:English
Subjects:
Analysis of Variance
Animals
Functional Laterality
Gene Expression Regulation
Genetic Vectors - administration & dosage
Genetic Vectors - genetics
Green Fluorescent Proteins - genetics
High-level
Humans
In vivo
Lentivirus
Lentivirus - genetics
Male
Neostriatum - cytology
Nerve Tissue Proteins - genetics
Nerve Tissue Proteins - metabolism
Neuron
Neurons - cytology
Neurons - metabolism
Promoter Regions, Genetic
Rats
Rats, Wistar
Specific
Tet-Off
Time Factors
Transduction, Genetic - methods
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Summary:We developed novel lentiviral vectors by using “Tet-Off system” and succeeded in achieving high-level and neuron-specific gene transduction in vivo. One week after viral injection into the rat neostriatum, the GFP expression was almost completely neuron-specific and about 40 times higher than the expression of a conventional lentiviral vector. High transcriptional activity and neuronal specificity were sustained for up to 8 weeks. Furthermore, neuronal processes of the infected neurons were efficiently visualized by adding a plasma membrane-targeting signal to GFP. These results suggest that the present method is valuable for strong gene transduction and clear visualization of neurons in vivo.
ISSN:0168-0102
1872-8111
DOI:10.1016/j.neures.2008.10.010