Rotor speed dependent sedimentation of circular and linear DNA

The sedimentation rate of large, linear DNA molecules has been shown to be rotor speed dependent (Rubenstein and Leighton, Biophys. Chem. 1 (1974)). In this communication we report the first studies designed to measure the rotor speed effect with a homogenous, linear viral DNA larger than bacterioph...

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Bibliographic Details
Published in:Biophysical chemistry 1979-05, Vol.9 (4), p.375-381
Main Authors: Ralston, Edward J., Schumaker, Verne N.
Format: Article
Language:English
Subjects:
Bacillus subtilis
Bacteriophages
DNA, Bacterial - isolation & purification
DNA, Circular - isolation & purification
DNA, Viral - isolation & purification
Escherichia coli
Mathematics
Molecular Weight
Ultracentrifugation
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Summary:The sedimentation rate of large, linear DNA molecules has been shown to be rotor speed dependent (Rubenstein and Leighton, Biophys. Chem. 1 (1974)). In this communication we report the first studies designed to measure the rotor speed effect with a homogenous, linear viral DNA larger than bacteriophage T2 DNA. We also report the first studies using a homogenous, circular episomal DNA of known molecular weight. For this circular DNA a small rotor speed effect, previously unsuspected, was discovered.
ISSN:0301-4622
1873-4200
DOI:10.1016/0301-4622(75)80052-4