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Liquid culture mass production of biocontrol nematodes, Heterorhabditis bacteriophora (Nematoda: Rhabditida): improved timing of dauer juvenile inoculation
Heterorhabditis bacteriophora is used in biological control of soil-borne insect pests in horticulture and turf. Mass production is carried out in monoxenic liquid cultures pre-incubated with the symbiont of the nematodes, the bacterium Photorhabdus luminescens, before nematode dauer juveniles (DJ)...
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Published in: | Applied microbiology and biotechnology 2004-06, Vol.64 (5), p.651-658 |
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description | Heterorhabditis bacteriophora is used in biological control of soil-borne insect pests in horticulture and turf. Mass production is carried out in monoxenic liquid cultures pre-incubated with the symbiont of the nematodes, the bacterium Photorhabdus luminescens, before nematode dauer juveniles (DJ) are inoculated. As a response to bacterial food signals, the DJ recover from the developmentally arrested dauer stage, grow to adults and produce DJ offspring. Variable DJ recovery after inoculation into cultures of P. luminescens often causes process failure due to low numbers of adult nematodes in the medium. In order to enhance DJ recovery, improve nematode population management and increase yields, the optimal timing for DJ inoculation was sought. The process parameter pH and respiration quotient (RQ) were recorded in order to test whether changes can be used to identify the best moment for DJ inoculation. When DJ were inoculated during the lag and early logarithmic growth phases of P. luminescens cultures, DJ recovery was low and almost no nematode reproduction was obtained. High populations of P. luminescens phase variants were recorded. Recovery and yields increased when DJ were inoculated during the latter log phase during which the RQ dropped to values |
doi_str_mv | 10.1007/s00253-003-1519-9 |
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Mass production is carried out in monoxenic liquid cultures pre-incubated with the symbiont of the nematodes, the bacterium Photorhabdus luminescens, before nematode dauer juveniles (DJ) are inoculated. As a response to bacterial food signals, the DJ recover from the developmentally arrested dauer stage, grow to adults and produce DJ offspring. Variable DJ recovery after inoculation into cultures of P. luminescens often causes process failure due to low numbers of adult nematodes in the medium. In order to enhance DJ recovery, improve nematode population management and increase yields, the optimal timing for DJ inoculation was sought. The process parameter pH and respiration quotient (RQ) were recorded in order to test whether changes can be used to identify the best moment for DJ inoculation. When DJ were inoculated during the lag and early logarithmic growth phases of P. luminescens cultures, DJ recovery was low and almost no nematode reproduction was obtained. High populations of P. luminescens phase variants were recorded. Recovery and yields increased when DJ were inoculated during the latter log phase during which the RQ dropped to values <0.8 and the pH reached a maximum. The highest DJ recovery and yields were observed in cultures that were inoculated during the late stationary growth phase. This period started with the increase of the pH after its distinct minimum at pH <8.0. Thus optimal timing for DJ inoculation can be defined through monitoring of the pH in the P. luminescens culture.</description><identifier>ISSN: 0175-7598</identifier><identifier>EISSN: 1432-0614</identifier><identifier>DOI: 10.1007/s00253-003-1519-9</identifier><identifier>PMID: 14727090</identifier><identifier>CODEN: AMBIDG</identifier><language>eng</language><publisher>Berlin: Springer</publisher><subject>Animals ; Bacteria ; Biological and medical sciences ; Biological control ; Biotechnology ; Fundamental and applied biological sciences. Psychology ; Heterorhabditis bacteriophora ; Horticulture ; Hydrogen-Ion Concentration ; Immunization ; Liquid culture ; Nematoda ; Nematodes ; Offspring ; Oxygen Consumption ; Pest control ; Photorhabdus - growth & development ; Photorhabdus luminescens ; Progeny ; Q1 ; Q2 ; Reproduction ; Respiration ; Rhabditida ; Rhabditida - growth & development ; Symbionts ; Symbiosis ; Turf</subject><ispartof>Applied microbiology and biotechnology, 2004-06, Vol.64 (5), p.651-658</ispartof><rights>2004 INIST-CNRS</rights><rights>Springer-Verlag 2004</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c423t-d183ee4339059c5bda475b80923eed0a99249ef8c543442f036e4ab49c513c0e3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.proquest.com/docview/621494013/fulltextPDF?pq-origsite=primo$$EPDF$$P50$$Gproquest$$H</linktopdf><linktohtml>$$Uhttps://www.proquest.com/docview/621494013?pq-origsite=primo$$EHTML$$P50$$Gproquest$$H</linktohtml><link.rule.ids>314,780,784,11688,27924,27925,36060,36061,44363,74895</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=15849132$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/14727090$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>JOHNIGK, S.-A</creatorcontrib><creatorcontrib>ECKE, F</creatorcontrib><creatorcontrib>POEHLING, M</creatorcontrib><creatorcontrib>EHLERS, R.-U</creatorcontrib><title>Liquid culture mass production of biocontrol nematodes, Heterorhabditis bacteriophora (Nematoda: Rhabditida): improved timing of dauer juvenile inoculation</title><title>Applied microbiology and biotechnology</title><addtitle>Appl Microbiol Biotechnol</addtitle><description>Heterorhabditis bacteriophora is used in biological control of soil-borne insect pests in horticulture and turf. Mass production is carried out in monoxenic liquid cultures pre-incubated with the symbiont of the nematodes, the bacterium Photorhabdus luminescens, before nematode dauer juveniles (DJ) are inoculated. As a response to bacterial food signals, the DJ recover from the developmentally arrested dauer stage, grow to adults and produce DJ offspring. Variable DJ recovery after inoculation into cultures of P. luminescens often causes process failure due to low numbers of adult nematodes in the medium. In order to enhance DJ recovery, improve nematode population management and increase yields, the optimal timing for DJ inoculation was sought. The process parameter pH and respiration quotient (RQ) were recorded in order to test whether changes can be used to identify the best moment for DJ inoculation. When DJ were inoculated during the lag and early logarithmic growth phases of P. luminescens cultures, DJ recovery was low and almost no nematode reproduction was obtained. High populations of P. luminescens phase variants were recorded. Recovery and yields increased when DJ were inoculated during the latter log phase during which the RQ dropped to values <0.8 and the pH reached a maximum. The highest DJ recovery and yields were observed in cultures that were inoculated during the late stationary growth phase. This period started with the increase of the pH after its distinct minimum at pH <8.0. Thus optimal timing for DJ inoculation can be defined through monitoring of the pH in the P. luminescens culture.</description><subject>Animals</subject><subject>Bacteria</subject><subject>Biological and medical sciences</subject><subject>Biological control</subject><subject>Biotechnology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Heterorhabditis bacteriophora</subject><subject>Horticulture</subject><subject>Hydrogen-Ion Concentration</subject><subject>Immunization</subject><subject>Liquid culture</subject><subject>Nematoda</subject><subject>Nematodes</subject><subject>Offspring</subject><subject>Oxygen Consumption</subject><subject>Pest control</subject><subject>Photorhabdus - growth & development</subject><subject>Photorhabdus luminescens</subject><subject>Progeny</subject><subject>Q1</subject><subject>Q2</subject><subject>Reproduction</subject><subject>Respiration</subject><subject>Rhabditida</subject><subject>Rhabditida - growth & development</subject><subject>Symbionts</subject><subject>Symbiosis</subject><subject>Turf</subject><issn>0175-7598</issn><issn>1432-0614</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>M0C</sourceid><recordid>eNp90cuKFDEUBuAgitOOPoAbCYI3sPTkUpfMTgZ1hEZBdB1SySknTVWlJ6kM-Cy-rCm6YMCFq8DhO38SfkKeMnjHANr3CYDXogIQFauZqtQ9smNS8AoaJu-THbC2rtpadWfkUUoHAMa7pnlIzphseQsKduTP3t9k76jN45Ij0smkRI8xuGwXH2YaBtr7YMO8xDDSGSezBIfpLb3CBWOI16Z3fvGJ9saWgQ_H6xANff31JM0F_b4RZ95cUD-V7Ft0dPGTn3-t8c5kjPSQb3H2I1I_h_IWs17-mDwYzJjwyXaek5-fPv64vKr23z5_ufywr6zkYqkc6wSiFEJBrWzdOyPbuu9A8TJ2YJTiUuHQ2VoKKfkAokFpelksExZQnJNXp9zytpuMadGTTxbH0cwYctKtbJjqlGqLfPl_yRRnwEWBz_-Bh5DjXH6hG86kksBWxE7IxpBSxEEfo59M_K0Z6LVgfSpYl4L1WrBWZefZFpz7Cd3dxtZoAS82YJI14xDNbH26c3UnFRNc_AU2m7AG</recordid><startdate>20040601</startdate><enddate>20040601</enddate><creator>JOHNIGK, S.-A</creator><creator>ECKE, F</creator><creator>POEHLING, M</creator><creator>EHLERS, R.-U</creator><general>Springer</general><general>Springer Nature B.V</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7T7</scope><scope>7WY</scope><scope>7WZ</scope><scope>7X7</scope><scope>7XB</scope><scope>87Z</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8FL</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BEZIV</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FRNLG</scope><scope>FYUFA</scope><scope>F~G</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K60</scope><scope>K6~</scope><scope>K9.</scope><scope>L.-</scope><scope>LK8</scope><scope>M0C</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PQBIZ</scope><scope>PQBZA</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>7X8</scope></search><sort><creationdate>20040601</creationdate><title>Liquid culture mass production of biocontrol nematodes, Heterorhabditis bacteriophora (Nematoda: Rhabditida): improved timing of dauer juvenile inoculation</title><author>JOHNIGK, S.-A ; ECKE, F ; POEHLING, M ; EHLERS, R.-U</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c423t-d183ee4339059c5bda475b80923eed0a99249ef8c543442f036e4ab49c513c0e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Animals</topic><topic>Bacteria</topic><topic>Biological and medical sciences</topic><topic>Biological control</topic><topic>Biotechnology</topic><topic>Fundamental and applied biological sciences. 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Academic</collection><jtitle>Applied microbiology and biotechnology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>JOHNIGK, S.-A</au><au>ECKE, F</au><au>POEHLING, M</au><au>EHLERS, R.-U</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Liquid culture mass production of biocontrol nematodes, Heterorhabditis bacteriophora (Nematoda: Rhabditida): improved timing of dauer juvenile inoculation</atitle><jtitle>Applied microbiology and biotechnology</jtitle><addtitle>Appl Microbiol Biotechnol</addtitle><date>2004-06-01</date><risdate>2004</risdate><volume>64</volume><issue>5</issue><spage>651</spage><epage>658</epage><pages>651-658</pages><issn>0175-7598</issn><eissn>1432-0614</eissn><coden>AMBIDG</coden><abstract>Heterorhabditis bacteriophora is used in biological control of soil-borne insect pests in horticulture and turf. Mass production is carried out in monoxenic liquid cultures pre-incubated with the symbiont of the nematodes, the bacterium Photorhabdus luminescens, before nematode dauer juveniles (DJ) are inoculated. As a response to bacterial food signals, the DJ recover from the developmentally arrested dauer stage, grow to adults and produce DJ offspring. Variable DJ recovery after inoculation into cultures of P. luminescens often causes process failure due to low numbers of adult nematodes in the medium. In order to enhance DJ recovery, improve nematode population management and increase yields, the optimal timing for DJ inoculation was sought. The process parameter pH and respiration quotient (RQ) were recorded in order to test whether changes can be used to identify the best moment for DJ inoculation. When DJ were inoculated during the lag and early logarithmic growth phases of P. luminescens cultures, DJ recovery was low and almost no nematode reproduction was obtained. High populations of P. luminescens phase variants were recorded. Recovery and yields increased when DJ were inoculated during the latter log phase during which the RQ dropped to values <0.8 and the pH reached a maximum. The highest DJ recovery and yields were observed in cultures that were inoculated during the late stationary growth phase. This period started with the increase of the pH after its distinct minimum at pH <8.0. Thus optimal timing for DJ inoculation can be defined through monitoring of the pH in the P. luminescens culture.</abstract><cop>Berlin</cop><pub>Springer</pub><pmid>14727090</pmid><doi>10.1007/s00253-003-1519-9</doi><tpages>8</tpages></addata></record> |
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subjects | Animals Bacteria Biological and medical sciences Biological control Biotechnology Fundamental and applied biological sciences. Psychology Heterorhabditis bacteriophora Horticulture Hydrogen-Ion Concentration Immunization Liquid culture Nematoda Nematodes Offspring Oxygen Consumption Pest control Photorhabdus - growth & development Photorhabdus luminescens Progeny Q1 Q2 Reproduction Respiration Rhabditida Rhabditida - growth & development Symbionts Symbiosis Turf |
title | Liquid culture mass production of biocontrol nematodes, Heterorhabditis bacteriophora (Nematoda: Rhabditida): improved timing of dauer juvenile inoculation |
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