Nicking endonucleases

Nicking endonucleases are a new type of enzymes. Like restriction endonucleases, they recognize short specific DNA sequence and cleave DNA at a fixed position relatively to the recognition sequence. However, unlike restriction endonucleases, nicking endonucleases cleave only one predetermined DNA st...

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Bibliographic Details
Published in:Biochemistry (Moscow) 2009-12, Vol.74 (13), p.1457-1466
Main Authors: Zheleznaya, L. A, Kachalova, G. S, Artyukh, R. I, Yunusova, A. K, Perevyazova, T. A, Matvienko, N. I
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Base Sequence
Biochemistry
Biomedical and Life Sciences
Biomedicine
Bioorganic Chemistry
Deoxyribonuclease I - chemistry
Deoxyribonuclease I - genetics
Deoxyribonuclease I - metabolism
Deoxyribonucleic acid
DNA
DNA Breaks, Single-Stranded
DNA Restriction Enzymes - genetics
DNA Restriction Enzymes - metabolism
Enzymes
Laboratories
Life Sciences
Microbiology
Models, Molecular
Molecular biology
Molecular Sequence Data
Nicking endonuclease
Nucleotide sequence
Protein Structure, Tertiary
Review
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Summary:Nicking endonucleases are a new type of enzymes. Like restriction endonucleases, they recognize short specific DNA sequence and cleave DNA at a fixed position relatively to the recognition sequence. However, unlike restriction endonucleases, nicking endonucleases cleave only one predetermined DNA strand. Until recently, nicking endonucleases were suggested to be naturally mutated restriction endonucleases which had lost their ability to dimerize and as a result the ability to cleave the second strand. We have shown that nicking endonucleases are one of the subunits of heterodimeric restriction endonucleases. Mechanisms used by various restriction endonucleases for double-stranded cleavage, designing of artificial nicking endonucleases on the basis of restriction endonucleases, and application of nicking endonucleases in molecular biology are reviewed.
ISSN:0006-2979
1608-3040
DOI:10.1134/S0006297909130033