Pseudonocardia rhizophila sp. nov., a novel actinomycete isolated from a rhizosphere soil

• Published in: Antonie van Leeuwenhoek 2010-06, Vol.98 (1), p.77-83
• Main Authors: Li, Jie, Zhao, Guo-Zhen, Huang, Hai-Yu, Zhu, Wen-Yong, Lee, Jae-Chan, Kim, Chang-Jin, Xu, Li-Hua, Zhang, Li-Xin, Li, Wen-Jun
• Format: Article
• Language: English
• Subjects: Actinomycetales - classification; Actinomycetales - genetics; Actinomycetales - isolation & purification; Actinomycetales - physiology; Actinomycetes; Bacteria; Bacterial Typing Techniques; Bacteriology; Base Composition; Biological and medical sciences; Biomedical and Life Sciences; China; Cluster Analysis; Data processing; Deoxyribonucleic acid; DNA; DNA, Bacterial - chemistry; DNA, Bacterial - genetics; DNA, Ribosomal - chemistry; DNA, Ribosomal - genetics; Fatty Acids - analysis; Fundamental and applied biological sciences. Psychology; Fungi; Genetics; genomics; Life Sciences; Medical Microbiology; Microbiology; Miscellaneous; Molecular Sequence Data; Mycelia; New species; Nucleic Acid Hybridization; Original Paper; Phylogeny; Physiology; Plant Roots - microbiology; Plant Sciences; Pseudonocardia; Rhizosphere; RNA, Ribosomal, 16S - genetics; rRNA 16S; Sequence Analysis, DNA; Sodium chloride; Soil; Soil Microbiology; Soil microorganisms; Soil Science & Conservation
• ISSN: 0003-6072; 1572-9699
• DOI: 10.1007/s10482-010-9431-7

During the course of our research on new actinobacterial sources, a novel actinomycete strain YIM 67013T was isolated from rhizosphere soil of Tripterygium wilfordii Hook. f. collected from Yunnan province, south-west China. The strain formed well-differentiated aerial and substrate mycelia, grew in the presence of up to 15% NaCl (optimum, 0-3% NaCl). Phylogenetic analysis based on 16S rRNA gene sequences showed that strain YIM 67013T belongs to the genus Pseudonocardia, with highest similarity to Pseudonocardia thermophila DSM 43832T (97.6%). Sequence similarities between strain YIM 67013T and the other Pseudonocardia species ranged from 96.6 to 93.2%. Key morphological and physiological characteristics as well as chemotaxonomic features of strain YIM 67013T were congruent with the description of the genus Pseudonocardia. The G+C content of the genomic DNA was 69.7 mol%. Based on comparative analysis of physiological, biochemical and chemotaxonomic data, including low DNA-DNA hybridization results, it is proposed that strain YIM 67013T represents a novel species of the genus Pseudonocardia, named Pseudonocardia rhizophila sp. nov. The type strain is YIM 67013T (= CCTCC AA 209043T = DSM 45381T).