Immunocytochemical localization of a calcium-binding protein in the rat duodenum

The cellular localization of the vitamin D-dependent calcium-binding protein (CaBP) in the duodenum of rat was studied using indirect immunofluorescence and immunoperoxidase-staining methods. Specific positive reaction product, indicative of the presence of CaBP, was exclusively located within the v...

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Bibliographic Details
Published in:Cell and tissue research 1979-03, Vol.197 (1), p.69-77
Main Authors: Marche, P, Le Guern, C, Cassier, P
Format: Article
Language:English
Subjects:
Animals
Carrier Proteins - isolation & purification
Cytoplasm - analysis
Duodenum - analysis
Fluorescent Antibody Technique
Histocytochemistry
Immunoenzyme Techniques
Intestinal Mucosa - analysis
Microvilli - analysis
Rats
S100 Calcium Binding Protein G - isolation & purification
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Summary:The cellular localization of the vitamin D-dependent calcium-binding protein (CaBP) in the duodenum of rat was studied using indirect immunofluorescence and immunoperoxidase-staining methods. Specific positive reaction product, indicative of the presence of CaBP, was exclusively located within the villous part of the duodenal mucosa. Moreover, CaBP was detected mainly within the supranuclear region of the cytoplasm of absorptive cells and also at the level of their basal laminae. CaBP was not demonstrable either in the nuclei or associated with the brush border membrane of absorptive cells. Also, CaBP was neither detectable in goblet cells nor in sub-epithelial layers. When the specific anti-CaBP antiserum was replaced by nonimmune rabbit serum or when it was preabsorbed on a CaBP-Sepharose conjugate, no positive immunostaining was seen. Together with recent biochemical data our observations agree well with the view that CaBP may act as an intracellular "buffer" by protecting the cell against too high Ca2+ concentrations.
ISSN:0302-766X
1432-0878
DOI:10.1007/BF00233554