Development of a complete set of disomic rape-radish chromosome-addition lines

• Published in: Euphytica 2008-07, Vol.162 (1), p.117-128
• Main Authors: Budahn, H, Schrader, O, Peterka, H
• Format: Article
• Language: English
• Subjects: addition lines; Agronomy. Soil science and plant productions; alien chromosome addition; backcrossing; Biological and medical sciences; Biomedical and Life Sciences; Biotechnology; Brassica napus; chromosome addition; chromosome transmission; Chromosomes; Fertility; fluorescence in situ hybridization; Fundamental and applied biological sciences. Psychology; Genetic markers; Genetics and breeding of economic plants; Hybridization; hybrids; intergeneric hybridization; Interspecific and intergeneric hybridization, introgressions; Life Sciences; monosomic addition lines; Plant breeding: fundamental aspects and methodology; Plant Genetics and Genomics; Plant Pathology; Plant Physiology; Plant reproduction; Plant Sciences; Pollen; Pollinators; radishes; Rape plants; rapeseed; Raphanus sativus; seed set
• ISSN: 0014-2336; 1573-5060
• DOI: 10.1007/s10681-007-9609-x

After crosses of multiple rape-radish chromosome additions with rape, nine different monosomic additions (2n = 4x = 38 + 1) of individual radish chromosomes, a-i, were isolated, having the genomic background of winter oil seed rape and radish cytoplasm. Extra chromosomes were identified with RAPD markers, specific for individual radish chromosomes. All radish-plasmic monosomic additions, except that of chromosome f, had low seed production due to pistilloid stamens. Rape cytoplasm was substituted by crossing the monosomic additions as pollinators to rape followed by reselection of the radish chromosome additions. The monosomic additions in rape cytoplasm showed normal male fertility. Average transmission rates of radish chromosomes via egg cells and pollen cells were 37% and 27%, respectively. Lowest male transmission was found for chromosomes e and f with 0.04 and 0.01. Monosomic additions were self-pollinated to produce disomic additions. Monosomic and disomic addition progenies were discriminated by a prescreening with quantitative double primer (dp) RAPDs and cytological confirmation of preselected candidates by FISH analysis using a Raphanus-specific probe. Usability of this complete set of nine disomic rape-radish additions (2n = 4x = 38 + 2) was discussed.