Cerebral Cortical Microfluorometry at Isosbestic Wavelengths for Correction of Vascular Artifact

Microfluorometric measurements of cerebral cortical mitochondrial respiration in vivo are obscured by hemodynamic and oximetric artifacts. Isosbestic fluorometry provides appropriate correction for these vascular phenomena and permits simultaneous evaluation of mitochondrial nicotinamide adenine din...

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Bibliographic Details
Published in:Science (American Association for the Advancement of Science) 1979-08, Vol.205 (4407), p.693-696
Main Authors: Kramer, Richard S., Pearlstein, Robert D.
Format: Article
Language:English
Subjects:
Absorption spectra
Animals
Blood volume
Body tissues
Brain Chemistry
Cerebral cortex
Cerebral Cortex - analysis
Dogs
Fluorescence
Fluorimetry
Fluorometry - instrumentation
Fluorometry - methods
Hemodynamics
Hemoglobins
Hemoglobins - analysis
Hypoxia, Brain - metabolism
Measurement
Metabolism
Mitochondria
Mitochondria - metabolism
NAD (Coenzyme)
NAD - analysis
NAD - metabolism
Nicotinamide adenine dinucleotide
Optical reflection
Oxidation-Reduction
Oxygen - blood
Physiological aspects
Rhodamines
Signal reflection
Wavelengths
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Summary:Microfluorometric measurements of cerebral cortical mitochondrial respiration in vivo are obscured by hemodynamic and oximetric artifacts. Isosbestic fluorometry provides appropriate correction for these vascular phenomena and permits simultaneous evaluation of mitochondrial nicotinamide adenine dinucleotide redox state, microcirculatory volume, and hemoglobin oxygenation.
ISSN:0036-8075
1095-9203
DOI:10.1126/science.223243