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Cerebral Cortical Microfluorometry at Isosbestic Wavelengths for Correction of Vascular Artifact

Microfluorometric measurements of cerebral cortical mitochondrial respiration in vivo are obscured by hemodynamic and oximetric artifacts. Isosbestic fluorometry provides appropriate correction for these vascular phenomena and permits simultaneous evaluation of mitochondrial nicotinamide adenine din...

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Published in:	Science (American Association for the Advancement of Science) 1979-08, Vol.205 (4407), p.693-696
Main Authors:	Kramer, Richard S., Pearlstein, Robert D.
Format:	Article
Language:	English
Subjects:	Absorption spectra Animals Blood volume Body tissues Brain Chemistry Cerebral cortex Cerebral Cortex - analysis Dogs Fluorescence Fluorimetry Fluorometry - instrumentation Fluorometry - methods Hemodynamics Hemoglobins Hemoglobins - analysis Hypoxia, Brain - metabolism Measurement Metabolism Mitochondria Mitochondria - metabolism NAD (Coenzyme) NAD - analysis NAD - metabolism Nicotinamide adenine dinucleotide Optical reflection Oxidation-Reduction Oxygen - blood Physiological aspects Rhodamines Signal reflection Wavelengths
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description	Microfluorometric measurements of cerebral cortical mitochondrial respiration in vivo are obscured by hemodynamic and oximetric artifacts. Isosbestic fluorometry provides appropriate correction for these vascular phenomena and permits simultaneous evaluation of mitochondrial nicotinamide adenine dinucleotide redox state, microcirculatory volume, and hemoglobin oxygenation.
doi_str_mv	10.1126/science.223243
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source	American Association for the Advancement of Science; JSTOR Archival Journals and Primary Sources Collection
subjects	Absorption spectra Animals Blood volume Body tissues Brain Chemistry Cerebral cortex Cerebral Cortex - analysis Dogs Fluorescence Fluorimetry Fluorometry - instrumentation Fluorometry - methods Hemodynamics Hemoglobins Hemoglobins - analysis Hypoxia, Brain - metabolism Measurement Metabolism Mitochondria Mitochondria - metabolism NAD (Coenzyme) NAD - analysis NAD - metabolism Nicotinamide adenine dinucleotide Optical reflection Oxidation-Reduction Oxygen - blood Physiological aspects Rhodamines Signal reflection Wavelengths
title	Cerebral Cortical Microfluorometry at Isosbestic Wavelengths for Correction of Vascular Artifact
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