Polymorphisms of DNA base-excision repair genes APE/Ref-1 and XRCC1 are not associated with the risk for Graves' disease

Oxidative stress has been implicated in etiopathogenesis of Graves' disease (GD). Increased lipid peroxidation and oxidative DNA damage have been found in GD patients. Oxidative DNA damage is mainly repaired by the base‐excision repair (BER) pathway. Polymorphisms in DNA‐repair genes have been...

Full description

Saved in:
Bibliographic Details
Published in:Cell biochemistry and function 2009-10, Vol.27 (7), p.462-467
Main Authors: Doğru-Abbasoğlu, Semra, Tanrıkulu, Sevda, Ademoğlu, Evin, Erbil, Yeşim, Özderya, Ayşenur, Karadağ, Berrin, Uysal, Müjdat
Format: Article
Language:English
Subjects:
Adolescent
Adult
Aged
APE/Ref-1
Case-Control Studies
Codon - genetics
DNA repair
DNA Repair - genetics
DNA-(Apurinic or Apyrimidinic Site) Lyase - genetics
DNA-Binding Proteins - genetics
Female
Gene Frequency
Genetic Predisposition to Disease
Graves Disease - enzymology
Graves Disease - genetics
Graves' disease
Haplotypes
Humans
Male
Middle Aged
Nucleic Acid Denaturation
polymorphism
Polymorphism, Single Nucleotide - genetics
X-ray Repair Cross Complementing Protein 1
XRCC1
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Oxidative stress has been implicated in etiopathogenesis of Graves' disease (GD). Increased lipid peroxidation and oxidative DNA damage have been found in GD patients. Oxidative DNA damage is mainly repaired by the base‐excision repair (BER) pathway. Polymorphisms in DNA‐repair genes have been associated with the increased risk of various diseases and could also be related to the etiology of GD. Therefore, we conducted a study including 197 patients with GD and age‐ and sex‐matched 303 healthy subjects to examine the role of single‐nucleotide polymorphisms of BER genes, APE/Ref‐1 (codon 148) and XRCC1 (codons 194 and 399) as a risk factor for GD. These polymorphisms were determined by quantitative real‐time PCR and melting curve analysis using LightCycler. No significant association was observed between the variant alleles of APE/Ref‐1 codon 148 [odds ratio (OR) = 0.89, 95% confidence interval (CI) = 0.69–1.17], XRCC1 codon 194 (OR = 1.24, 95% CI = 0.79–1.94), and XRCC1 codon 399 (OR = 1.12, 95% CI = 0.86–1.46) and GD. These preliminary results suggest that APE/Ref‐1 (codon 148) and XRCC1 (codons 194 and 399) polymorphisms are not significant risk factors for developing GD. Copyright © 2009 John Wiley & Sons, Ltd.
ISSN:0263-6484
1099-0844
DOI:10.1002/cbf.1595