Effect of the herbicide glyphosate on glyphosate-tolerant maize rhizobacterial communities: a comparison with pre-emergency applied herbicide consisting of a combination of acetochlor and terbuthylazine

A comparison was made of the effect of glyphosate (Roundup®Plus), a post-emergency applied herbicide, and of Harness®GTZ, a pre-emergency applied herbicide, on the rhizobacterial communities of genetically modified NK603 glyphosate-tolerant maize. The potential effect was monitored by direct amplifi...

Full description

Saved in:
Bibliographic Details
Published in:Environmental microbiology 2010-04, Vol.12 (4), p.1021-1030
Main Authors: Barriuso, Jorge, Marín, Silvia, Mellado, Rafael P
Format: Article
Language:English
Subjects:
DNA, Bacterial - genetics
Genome, Bacterial
Glycine - analogs & derivatives
Glycine - pharmacology
Glyphosate
Herbicides - pharmacology
Oligonucleotide Array Sequence Analysis
Plants, Genetically Modified - microbiology
RNA, Ribosomal, 16S - genetics
Sequence Alignment
Sequence Analysis, RNA
Soil - analysis
Soil Microbiology
Soil Pollutants - pharmacology
Streptomyces coelicolor
Streptomyces coelicolor - drug effects
Streptomyces coelicolor - genetics
Toluidines - pharmacology
Triazines - pharmacology
Zea mays
Zea mays - microbiology
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:A comparison was made of the effect of glyphosate (Roundup®Plus), a post-emergency applied herbicide, and of Harness®GTZ, a pre-emergency applied herbicide, on the rhizobacterial communities of genetically modified NK603 glyphosate-tolerant maize. The potential effect was monitored by direct amplification, cloning and sequencing of soil DNA encoding 16S rRNA, rhizobacterial DNA hybridization to commercially available genome-wide microarrays from the soil bacterium Streptomyces coelicolor, and high-throughput DNA pyrosequencing of the bacterial DNA coding for 16S rRNA hypervariable V6 region. The results obtained strongly suggest that both herbicides do in fact affect the maize rhizobacterial communities, glyphosate being, to a great extent, the environmentally less aggressive herbicide.
ISSN:1462-2912
1462-2920
DOI:10.1111/j.1462-2920.2009.02146.x