Identification of avermectin-high-producing strains by high-throughput screening methods

Avermectins produced by Streptomyces avermitilis are potent against a broad spectrum of nematode and arthropod parasites with low-level side effects on the host organisms. This study was designed to investigate a high-throughput screening strategy for the efficient identification of avermectin high-...

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Bibliographic Details
Published in:Applied microbiology and biotechnology 2010-01, Vol.85 (4), p.1219-1225
Main Authors: Gao, Hong, Liu, Mei, Zhou, Xianlong, Liu, Jintao, Zhuo, Ying, Gou, Zhongxuan, Xu, Bing, Zhang, Wenquan, Liu, Xiangyang, Luo, Aiqun, Zheng, Chuansen, Chen, Xiaoping, Zhang, Lixin
Format: Article
Language:English
Subjects:
Accuracy
Arthropoda
Biological and medical sciences
Biomedical and Life Sciences
Biotechnology
Chemicals
Chromatography, High Pressure Liquid
Fermentation
Fundamental and applied biological sciences. Psychology
High-Throughput Screening Assays
Industrial Microbiology
Ivermectin - analogs & derivatives
Ivermectin - analysis
Ivermectin - metabolism
Life Sciences
Methods
Microbial Genetics and Genomics
Microbiology
Nematoda
Parasites
Reproducibility
Reproducibility of Results
Streptomyces - genetics
Streptomyces - growth & development
Streptomyces - isolation & purification
Streptomyces - metabolism
Streptomyces avermitilis
Studies
Ultraviolet Rays
Yeast
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Summary:Avermectins produced by Streptomyces avermitilis are potent against a broad spectrum of nematode and arthropod parasites with low-level side effects on the host organisms. This study was designed to investigate a high-throughput screening strategy for the efficient identification of avermectin high-yield strains. The production protocol was miniaturized in 96 deep-well microplates. UV absorbance at 245 nm was used to monitor avermectin production. A good correlation between fermentation results in both 96 deep-well microplates and conventional Erlenmeyer flasks was observed. With this protocol, the production of avermectins was determined in less than 10 min for a full plate without compromising accuracy. The high-yield strain selected through this protocol was also tested in 360 m³ batch fermentation with 1.6-fold improved outcome. Thus, the development of this protocol is expected to accelerate the selection of superior avermectin-producing strains.
ISSN:0175-7598
1432-0614
DOI:10.1007/s00253-009-2345-5