Response of alfalfa (Medicago sativa L.) to single and mixed inoculation with phosphate-solubilizing bacteria and Sinorhizobium meliloti

The objectives of this work were to phenotypically and genetically characterize alfalfa rhizosphere bacteria and to evaluate the effect of single or mixed inoculation upon nodulation and biological nitrogen fixation. Thirty-two strains showed tricalcium phosphate solubilization ability, and two of t...

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Bibliographic Details
Published in:Biology and fertility of soils 2010-02, Vol.46 (2), p.185-190
Main Authors: Guiñazú, Lorena Belén, Andrés, Javier Alberto, Del Papa, María Florencia, Pistorio, Mariano, Rosas, Susana Beatriz
Format: Article
Language:English
Subjects:
Agriculture
Alfalfa
Bacillus
Bacteria
Biomedical and Life Sciences
Calcium phosphates
Flowers & plants
Genotype & phenotype
Life Sciences
Medicago sativa
Nitrogen
Nitrogen fixation
Pseudomonas
Rhizosphere
Short Communication
Sinorhizobium meliloti
Soil Science & Conservation
Symbiosis
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Summary:The objectives of this work were to phenotypically and genetically characterize alfalfa rhizosphere bacteria and to evaluate the effect of single or mixed inoculation upon nodulation and biological nitrogen fixation. Thirty-two strains showed tricalcium phosphate solubilization ability, and two of them caused bigger or equal solubilization halos than the control strain P. putida SP22. The comparison of the 16S ribosomal DNA sequences indicated that these strains are phylogenetically related to Bacillus spp. and Pseudomonas spp. A beneficial effect of both isolates on alfalfa growth was observed in coinoculation assays. Pseudomonas sp. FM7d caused a significant increase in root and shoot dry weight, length, and surface area of roots, number, and symbiotic properties of alfalfa plants. The plants coinoculated with Sinorhizobium meliloti B399 and the Bacillus sp. M7c showed significant increases in the measured parameters. Our results indicating that strains Pseudomonas sp. FM7d and Bacillus sp. M7c can be considered for the formulation of new inoculants.
ISSN:0178-2762
1432-0789
DOI:10.1007/s00374-009-0408-5