Growth promotion of cucumber by pure cultures of gibberellin-producing Phoma sp. GAH7

The beneficial effects of plant growth promoting fungi (PGPF) on plant growth and development are well documented. However, limited information is available on gibberellin (GA) production capacity of PGPF of endophytic origin. In current study, 11 fungal endophytes were isolated from cucumber roots...

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Bibliographic Details
Published in:World journal of microbiology & biotechnology 2010-05, Vol.26 (5), p.889-894
Main Authors: Hamayun, Muhammad, Khan, Sumera Afzal, Khan, Abdul Latif, Tang, Dong-Sheng, Hussain, Javid, Ahmad, Bashir, Anwar, Yasir, Lee, In-Jung
Format: Article
Language:English
Subjects:
Agriculture
Applied Microbiology
Bioassays
Biochemistry
Biological and medical sciences
Biomedical and Life Sciences
Biotechnology
Cucumbers
Cucumis sativus
Endophytes
Environmental Engineering/Biotechnology
Fertilizers
Filtrate
Fundamental and applied biological sciences. Psychology
Fungi
Fusarium fujikuroi
Hormones
Horticulture
Life Sciences
Loam soils
Microbiology
Original Paper
Oryza sativa
Pathogens
Phoma
Phylogenetics
Plant growth
Production capacity
Ribosomal DNA
Rice
Seeds
Studies
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Summary:The beneficial effects of plant growth promoting fungi (PGPF) on plant growth and development are well documented. However, limited information is available on gibberellin (GA) production capacity of PGPF of endophytic origin. In current study, 11 fungal endophytes were isolated from cucumber roots and then screened on Waito-C rice, in order to identify plant growth promoting fungal strains. The fungal isolate GAH7 provided the maximum shoot length (11.3 cm) in comparison to control treatment (7.8 cm). In a separate experiment, bioassay of GAH7 significantly promoted growth attributes of cucumber. The GAH7 culture filtrate (CF) was found to contain physiologically active gibberellins in higher concentrations (GA₁, 0.81 ng/ml; GA₃, 4.34 ng/ml and GA₄, 9.31 ng/ml) in conjunction with physiologically inactive GA₉ (0.74 ng/ml), GA₁₅ (0.97 ng/ml), GA₁₉ (1.67 ng/ml) and GA₂₀ (0.46 ng/ml). Isolate GAH7 produced higher amounts of GA₃, GA₄, GA₉ and GA₁₉ than wild type Fusarium fujikuroi, which was used as control for GA production. Gibberellins were analyzed through gas chromatograph/mass spectrometer (GC/MS) with selected ion monitoring (SIM). The fungal isolate GAH7 was later identified as a new strain of Phoma on the basis of sequence homology (99%) and phylogenetic analysis of 18S rDNA sequence.
ISSN:0959-3993
1573-0972
DOI:10.1007/s11274-009-0248-3