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Argonautes ALG-3 and ALG-4 are required for spermatogenesis-specific 26G-RNAs and thermotolerant sperm in Caenorhabditis elegans
Gametogenesis is a thermosensitive process in numerous metazoans, ranging from worms to man. In Caenorhabditis elegans, a variety of RNA-binding proteins that associate with germ-line nuage (P granules), including the Piwi-clade argonaute PRG-1, have been implicated in maintaining fertility at eleva...
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| Published in: | Proceedings of the National Academy of Sciences - PNAS 2010-02, Vol.107 (8), p.3588-3593 |
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| container_issue | 8 |
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| container_title | Proceedings of the National Academy of Sciences - PNAS |
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| creator | Conine, Colin C Batista, Pedro J Gu, Weifeng Claycomb, Julie M Chaves, Daniel A Shirayama, Masaki Mello, Craig C |
| description | Gametogenesis is a thermosensitive process in numerous metazoans, ranging from worms to man. In Caenorhabditis elegans, a variety of RNA-binding proteins that associate with germ-line nuage (P granules), including the Piwi-clade argonaute PRG-1, have been implicated in maintaining fertility at elevated temperature. Here we describe the role of two AGO-class paralogs, alg-3 (T22B3.2) and alg-4 (ZK757.3), in promoting thermotolerant male fertility. A rescuing GFP::alg-3 transgene is localized to P granules beginning at the late pachytene stage of male gametogenesis. alg-3/4 double mutants lack a subgroup of small RNAs, the 26G-RNAs which target and appear to down-regulate numerous spermatogenesis-expressed mRNAs. These findings add to a growing number of AGO pathways required for thermotolerant fertility in C. elegans and support a model in which AGOs and their small RNA cofactors function to promote robustness in gene-expression networks. |
| doi_str_mv | 10.1073/pnas.0911685107 |
| format | article |
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In Caenorhabditis elegans, a variety of RNA-binding proteins that associate with germ-line nuage (P granules), including the Piwi-clade argonaute PRG-1, have been implicated in maintaining fertility at elevated temperature. Here we describe the role of two AGO-class paralogs, alg-3 (T22B3.2) and alg-4 (ZK757.3), in promoting thermotolerant male fertility. A rescuing GFP::alg-3 transgene is localized to P granules beginning at the late pachytene stage of male gametogenesis. alg-3/4 double mutants lack a subgroup of small RNAs, the 26G-RNAs which target and appear to down-regulate numerous spermatogenesis-expressed mRNAs. 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| subjects | Animals Binding sites Biological Sciences Caenorhabditis elegans Caenorhabditis elegans - genetics Caenorhabditis elegans - physiology Caenorhabditis elegans Proteins - genetics Caenorhabditis elegans Proteins - metabolism Cell division Fertility - genetics Fertility - physiology Gametogenesis Gene expression Gene expression regulation Germ cells Hot Temperature Male Male animals Messenger RNA Metazoa Mutation Nematodes Proteins Ribonucleic acid RNA RNA, Small Interfering - biosynthesis RNA, Small Interfering - genetics RNA-Binding Proteins - genetics RNA-Binding Proteins - metabolism Spermatids Spermatogenesis Spermatogenesis - genetics Spermatogenesis - physiology Spermatozoa Spermatozoa - metabolism |
| title | Argonautes ALG-3 and ALG-4 are required for spermatogenesis-specific 26G-RNAs and thermotolerant sperm in Caenorhabditis elegans |
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