Characterization of a Bombyx mori nucleopolyhedrovirus mutant lacking both fp25K and p35

Recent studies have shown that dual mutations in fp25K and p35 of Autographa californica nucleopolyhedrovirus (AcMNPV) result in a typical apoptotic infection on Trichoplusia ni cells, suggesting the involvement of FP25K on NPV-induced apoptosis. To examine the effect of fp25K deletion on Bombyx mor...

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Bibliographic Details
Published in:Virus genes 2010-08, Vol.41 (1), p.144-148
Main Authors: Nakanishi, Tadashi, Shimada, Toru, Katsuma, Susumu
Format: Article
Language:English
Subjects:
Animals
apoptosis
Autographa californica
Baculoviridae
Biomedical and Life Sciences
Biomedicine
BmNPV
Bombyx - virology
Bombyx mori NPV
Few polyhedra
Gene Deletion
Genome, Viral
Larva - virology
Medical Microbiology
Mutation
Nuclear polyhedrosis virus
Nucleopolyhedrovirus - genetics
Nucleopolyhedrovirus - metabolism
p35
Plant Sciences
Trichoplusia ni
Viral Proteins - genetics
Viral Proteins - metabolism
Virology
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Summary:Recent studies have shown that dual mutations in fp25K and p35 of Autographa californica nucleopolyhedrovirus (AcMNPV) result in a typical apoptotic infection on Trichoplusia ni cells, suggesting the involvement of FP25K on NPV-induced apoptosis. To examine the effect of fp25K deletion on Bombyx mori NPV (BmNPV)-induced apoptosis, we generated a BmNPV mutant, fp-p35D, in which both fp25K and p35 genes are deleted from the genome, and compared its phenotype with wild-type (T3), fp25K-deleted (fp-null), and p35-deleted (p35D) BmNPVs. In BmN cells, p35D, but not T3 or fp-null, caused apoptosis with caspase-3 activation. Infection with fp-p35D also resulted in caspase-3 activation, but the level was comparable to that of p35D. Also, we did not observe any apoptotic responses in hemocytes from larvae infected with p35D or fp-p35D. These results indicate that unlike AcMNPV, deletion of fp25K does not affect the pathway of p35D-induced apoptosis of BmN cells and B. mori larvae.
ISSN:0920-8569
1572-994X
DOI:10.1007/s11262-010-0492-5