Adipokines and Cytokines in Human Pancreatic Juice: Unraveling the Local Pancreatic Inflammatory Milieu

• Published in: Digestive diseases and sciences 2010-07, Vol.55 (7), p.2108-2112
• Main Authors: Dalbec, Kathryn M, Max Schmidt, C, Wade, Terence E, Wang, Sue, Swartz-Basile, Deborah A, Pitt, Henry A, Zyromski, Nicholas J
• Format: Article
• Language: English
• Subjects: Adipokines - analysis; Adipokines - metabolism; Adiponectin - analysis; Adiponectin - metabolism; Adult; Aged; Analysis; Biochemistry; Biological and medical sciences; Cholangiopancreatography, Endoscopic Retrograde - methods; Cytokines - analysis; Cytokines - metabolism; Disease Progression; Enzyme-Linked Immunosorbent Assay; Enzymes; Feeding. Feeding behavior; Female; Fundamental and applied biological sciences. Psychology; Gastroenterology; Hepatology; Humans; Inflammation Mediators - analysis; Inflammation Mediators - metabolism; Insulin - analysis; Insulin - metabolism; Interleukins; Leptin; Leptin - analysis; Leptin - metabolism; Linear Models; Male; Medicine; Medicine & Public Health; Middle Aged; Obesity; Oncology; Original Article; Pancreatic Function Tests; Pancreatic Juice - chemistry; Pancreatitis - diagnosis; Predictive Value of Tests; Probability; Prognosis; Risk Assessment; Sampling Studies; Severity of Illness Index; Transplant Surgery; Vertebrates: anatomy and physiology, studies on body, several organs or systems
• ISSN: 0163-2116; 1573-2568
• DOI: 10.1007/s10620-009-0977-z

Background Differential adipokine expression in obesity influences the inflammatory milieu, and may explain in part obesity's negative impact on pancreatic disease. Pancreatic juice analysis may provide a good means to evaluate the local pancreatic inflammatory milieu. The presence of adipokines in pancreatic juice is unknown. Aims This proof-of-concept study was designed to determine the presence of adipokines and cytokines in human pancreatic juice. Methods With institutional review board approval, pancreatic juice was obtained from ten patients with a broad range of diagnoses at the time of endoscopic retrograde cholangiopancreatography. Pancreatic juice was assayed using enzyme-linked immunosorbent assay (ELISA) for insulin, the proinflammatory adipokine leptin, the anti-inflammatory adipokine adiponectin, and the inflammatory mediators interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α), and monocyte chemoattractant protein-1 (MCP-1). Correlation between adipokine and inflammatory mediator expression was determined by linear regression analysis. Data are presented as mean ± standard error of the mean (SEM); P < 0.05 was considered statistically significant. Results Leptin (0.16 ± 0.05 ng/ml) and adiponectin (0.63 ± 0.02 μg/ml) were both present, as were the inflammatory mediators IL-6 (112.6 ± 28.1 pg/ml), TNF-α (49.0 ± 18.8 pg/ml), and MCP-1 (32.2 ± 0.9 pg/ml). Paradoxically, the expression of the anti-inflammatory adipokine adiponectin correlated strongly with that of the proinflammatory cytokine IL-6 (R ² = 0.98, P < 0.001). Conclusions This report is the first to describe adipokine expression in human pancreatic juice. Human pancreatic juice inflammatory mediators and adipokines may provide an important measurement of the local pancreatic inflammatory milieu.