Development, Characterization and Cross Species Amplification of Polymorphic Microsatellite Markers from Expressed Sequence Tags of Turmeric (Curcuma longa L.)

• Published in: Molecular biotechnology 2010-02, Vol.44 (2), p.140-147
• Main Authors: Siju, S., Dhanya, K., Syamkumar, S., Sasikumar, B., Sheeja, T. E., Bhat, A. I., Parthasarathy, V. A.
• Format: Article
• Language: English
• Subjects: Biochemistry; Biological and medical sciences; Biological Techniques; Biotechnology; Cell Biology; Chemistry; Chemistry and Materials Science; Curcuma - genetics; Curcuma longa; DNA, Plant - genetics; Electrophoresis; Expressed Sequence Tags; Flowers & plants; Fundamental and applied biological sciences. Psychology; Genetic diversity; Human Genetics; Microsatellite Repeats - genetics; Polymerase Chain Reaction; Polymorphism; Protein Science; Satellite DNA
• ISSN: 1073-6085; 1559-0305
• DOI: 10.1007/s12033-009-9222-4

Expressed sequence tags (ESTs) from turmeric ( Curcuma longa L.) were used for the screening of type and frequency of Class I (hypervariable) simple sequence repeats (SSRs). A total of 231 microsatellite repeats were detected from 12,593 EST sequences of turmeric after redundancy elimination. The average density of Class I SSRs accounts to one SSR per 17.96 kb of EST. Mononucleotides were the most abundant class of microsatellite repeat in turmeric ESTs followed by trinucleotides. A robust set of 17 polymorphic EST–SSRs were developed and used for evaluating 20 turmeric accessions. The number of alleles detected ranged from 3 to 8 per loci. The developed markers were also evaluated in 13 related species of C. longa confirming high rate (100%) of cross species transferability. The polymorphic microsatellite markers generated from this study could be used for genetic diversity analysis and resolving the taxonomic confusion prevailing in the genus.