Development of cell culture system from the giant freshwater prawn Macrobrachium rosenbergii (de Man)

A new cell culture system (MRH) was developed for the first time from 2 months old freshwater prawn, Macrobrachium rosenbergii. Primary cultures were developed from heart tissues by explant culture technique. Cell outgrowth was obtained from the heart explant after 14 days of explant culture. The cu...

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Bibliographic Details
Published in:Molecular biology reports 2010-04, Vol.37 (4), p.2043-2048
Main Authors: Goswami, Mukunda, Lakra, Wazir S, Rajaswaminathan, T, Rathore, Gourav
Format: Article
Language:English
Subjects:
Animal Anatomy
Animal Biochemistry
Animal diseases
Animals
Aquaculture
Biomedical and Life Sciences
Cell culture
Cell Culture Techniques - methods
Cell Proliferation
Crustaceans
Fibroblasts - cytology
Fresh Water
Freshwater
Histology
Life Sciences
Macrobrachium rosenbergii
Microscopy, Phase-Contrast
Molecular Sequence Data
Morphology
Palaemonidae - cytology
Pathology
Serum
Temperature
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Summary:A new cell culture system (MRH) was developed for the first time from 2 months old freshwater prawn, Macrobrachium rosenbergii. Primary cultures were developed from heart tissues by explant culture technique. Cell outgrowth was obtained from the heart explant after 14 days of explant culture. The culture medium used was Leibovitz-15 supplemented with 20% Fetal Bovine Serum along with 1% prawn hemolymph serum, 0.1% glucose, 0.5% NaCl and antibiotics (Penicillin 10,000 Units ml⁻¹, Streptomycin 10,000 μg ml⁻¹, Amphotericin B 500 mg ml⁻¹) with a final osmomolality of 470-550 mmol kg⁻¹. The pH of the growth medium found suitable for the growth of the cells was 7.20. The viability of cells was found to be 60% when revived after a month of storage in liquid nitrogen.
ISSN:0301-4851
1573-4978
DOI:10.1007/s11033-009-9659-3