Carbamoylases: characteristics and applications in biotechnological processes

Enzymatic kinetic resolution is a widely used biotechnological tool for the production of enantiomerically pure/enriched compounds. This technique takes advantage of the enantioselectivity or enantiospecificity of an enzyme for one of the enantiomers of a racemic substrate to isolate the desired iso...

Full description

Saved in:
Bibliographic Details
Published in:Applied microbiology and biotechnology 2010, Vol.85 (3), p.441-458
Main Authors: Martínez-Rodríguez, Sergio, Martínez-Gómez, Ana Isabel, Rodríguez-Vico, Felipe, Clemente-Jiménez, Josefa María, Las Heras-Vázquez, Francisco Javier
Format: Article
Language:English
Subjects:
Amidohydrolases - chemistry
Amidohydrolases - metabolism
Amino Acid Sequence
Amino acids
Amino Acids - metabolism
Biological and medical sciences
Biomedical and Life Sciences
Biotechnology
Biotechnology - methods
Enzymes
Fundamental and applied biological sciences. Psychology
Kinetics
Life Sciences
Microbial Genetics and Genomics
Microbiology
Mini-Review
Models, Molecular
Molecular Sequence Data
Protein Structure, Tertiary
Sequence Homology
Stereoisomerism
Studies
Substrate Specificity
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Enzymatic kinetic resolution is a widely used biotechnological tool for the production of enantiomerically pure/enriched compounds. This technique takes advantage of the enantioselectivity or enantiospecificity of an enzyme for one of the enantiomers of a racemic substrate to isolate the desired isomer. N-Carbamoyl-d- and l-amino acid amidohydrolases (d- and l-carbamoylases) are model enzymes for this procedure due to their strict enantiospecificity. Carbamoylase-based kinetic resolution of amino acids has been applied for the last three decades, allowing the production of optically pure d- or l-amino acids. Furthermore, this enzyme has become crucial in the industrially used multienzymatic system known as “Hydantoinase Process,” where the kinetic resolution produced by coupling an enantioselective hydantoinase and the enantiospecific carbamoylase is enhanced by the enzymatic/chemical dynamic kinetic resolution of the low-rate hydrolyzed substrate. This review outlines the properties of d- and l-carbamoylases, emphasizing their biochemical/structural characteristics and their biotechnological applications. It also pinpoints new applications for the exploitation of carbamoylases over the forthcoming years.
ISSN:0175-7598
1432-0614
DOI:10.1007/s00253-009-2250-y