A novel mosquitocidal Bacillus thuringiensis strain LLP29 isolated from the phylloplane of Magnolia denudata

Eleven Bacillus thuringiensis isolates were recovered from phylloplanes of Magnolia denudata, a specific source of new strains of B. thuringiensis. Among these, a new strain, LLP29, was found to be most toxic to mosquitoes based on the results of preliminary toxicity analysis. Phase contrast microsc...

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Bibliographic Details
Published in:Microbiological research 2010-02, Vol.165 (2), p.133-141
Main Authors: Zhang, Lingling, Huang, Enjiong, Lin, Jing, Gelbič, Ivan, Zhang, Qunlin, Guan, Yi, Huang, Tianpei, Guan, Xiong
Format: Article
Language:English
Subjects:
Aedes - microbiology
Aedes albopictus
Animals
Bacillus thuringiensis
Bacillus thuringiensis - classification
Bacillus thuringiensis - isolation & purification
Bacillus thuringiensis - pathogenicity
Bacterial Toxins - genetics
Bacterial Toxins - metabolism
Base Sequence
Cloning, Molecular
Culex - microbiology
Culex quinquefasciatus
cyt1
DNA, Bacterial - chemistry
DNA, Bacterial - genetics
Insect Control - methods
Insecticides - metabolism
Lethal Dose 50
Magnolia
Magnolia - microbiology
Microscopy, Phase-Contrast
Molecular Sequence Data
Mosquito
Pest Control, Biological - methods
Phylloplane
Plant Leaves - microbiology
Polymerase Chain Reaction
Sequence Alignment
Sequence Analysis, DNA
Survival Analysis
Toxicity
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Summary:Eleven Bacillus thuringiensis isolates were recovered from phylloplanes of Magnolia denudata, a specific source of new strains of B. thuringiensis. Among these, a new strain, LLP29, was found to be most toxic to mosquitoes based on the results of preliminary toxicity analysis. Phase contrast microscopy, mosquitocidal activity, polymerase chain reaction (PCR) analysis and parasporal inclusion were performed to learn more about the characteristics of this novel mosquitocidal isolate. The LC 50 values of LLP29 against Aedes albopictus and Culex quinquefasciatus were 0.33 and 0.04 ng of protein/ml, respectively. The cyt1 gene, which encodes the Cyt protein that is toxic to mosquitoes, was subsequently detected, cloned, sequenced and expressed in acrystalliferous Bt HD73 Cry −. The results indicated that it might be a member of the cyt1Aa gene group. The novel strain LLP29 appears to be a new subspecies of B. thuringiensis and should prove useful in the control of mosquitoes and mosquito-borne diseases.
ISSN:0944-5013
1618-0623
DOI:10.1016/j.micres.2009.03.002