Microbial community of acetate utilizing denitrifiers in aerobic granules

The aerobic sludge granules cultivated at high organic loading rates could effectively convert 100-700 mg l⁻¹ nitrite to nitrogen gas with 400 or 1,200 mg l⁻¹ dosed acetate. The denitrifying microbial community structure of the so-cultivated granules was investigated by 16S rRNA gene sequences and l...

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Bibliographic Details
Published in:Applied microbiology and biotechnology 2010, Vol.85 (3), p.753-762
Main Authors: Adav, Sunil S, Lee, Duu-Jong, Lai, J. Y
Format: Article
Language:English
Subjects:
Bacteria
Bacteria, Aerobic - classification
Bacteria, Aerobic - genetics
Bacteria, Aerobic - metabolism
Biodiversity
Biological and medical sciences
Biomedical and Life Sciences
Biotechnology
Chemical engineering
Chemical oxygen demand
Cloning
Cluster Analysis
Comamonadaceae
Community structure
Denitrification
DNA, Bacterial - chemistry
DNA, Bacterial - genetics
DNA, Ribosomal - chemistry
DNA, Ribosomal - genetics
Environmental Biotechnology
Fundamental and applied biological sciences. Psychology
Gene amplification
Genes
Hybridization
In Situ Hybridization, Fluorescence
Lasers
Life Sciences
Microbial Genetics and Genomics
Microbiology
Microscopy, Confocal
Microscopy, Electron, Scanning
Molecular Sequence Data
Nitrates
Nitric oxide
Nitrites - metabolism
Nitrogen - metabolism
Nitrous oxide
Organic loading
Phylogeny
Reactors
Ribonucleic acid
RNA
RNA, Ribosomal, 16S - genetics
Sequence Analysis, DNA
Sewage - microbiology
Sludge
Staining and Labeling
Studies
Water treatment
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Description
Summary:The aerobic sludge granules cultivated at high organic loading rates could effectively convert 100-700 mg l⁻¹ nitrite to nitrogen gas with 400 or 1,200 mg l⁻¹ dosed acetate. The denitrifying microbial community structure of the so-cultivated granules was investigated by 16S rRNA gene sequences and localized using fluorescence in situ hybridization (FISH). The 16S rRNA gene phylotypes in the clone library and FISH probes used exhibited high diversity among the bacteria and denitrifying communities, with the members of Betaproteobacteria predominant that were closely related to families Comamonadaceae, Nitrosomonadaceae, Alcaligenaceae, and Rhodocyclaceae. The confocal laser scanning microscope and staining test revealed that active microbial community principally distributed at 200-250 μm beneath the outer surface, embedded in extracellular polymeric substances.
ISSN:0175-7598
1432-0614
DOI:10.1007/s00253-009-2263-6