Study of the Olfactory Epithelium in the Developing Sturgeon. Characterization of the Crypt Cells

In acipenserids, crypt cells (CCs) have only been observed in juvenile specimens, and it has not been clarified whether they differentiate along with olfactory receptor neurons (ORNs) during the lecithotrophic stage or during later development stages. Furthermore, no detailed optical microscopy (OM)...

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Bibliographic Details
Published in:Chemical senses 2010-02, Vol.35 (2), p.147-156
Main Authors: Camacho, Susana, Ostos-Garrido, M.V., Domezain, A., Carmona, R.
Format: Article
Language:English
Subjects:
A. naccarii
Acipenser naccarii
Animals
Biological and medical sciences
Cell Differentiation
Cell Nucleus - physiology
Cilia - ultrastructure
crypt cells
Fishes - anatomy & histology
Fishes - growth & development
Fundamental and applied biological sciences. Psychology
Microscopy, Confocal
Microscopy, Electron, Scanning
Microscopy, Electron, Transmission
Olfaction. Taste
Olfactory Mucosa - cytology
Olfactory Mucosa - ultrastructure
olfactory organ
olfactory receptor neurons
Olfactory Receptor Neurons - cytology
Olfactory Receptor Neurons - ultrastructure
Perception
Psychology. Psychoanalysis. Psychiatry
Psychology. Psychophysiology
sturgeon
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Summary:In acipenserids, crypt cells (CCs) have only been observed in juvenile specimens, and it has not been clarified whether they differentiate along with olfactory receptor neurons (ORNs) during the lecithotrophic stage or during later development stages. Furthermore, no detailed optical microscopy (OM) or electron microscopy study on the development of CCs has been published to date. In the present study, we used OM and electron microscopy to follow the development of CCs in Acipenser naccarii from hatching to the establishment of exogenous feeding. Based on these observations, we can affirm that CCs are present from the first few posthatching (PH) days. CCs appear with their nucleus close to the basal lamina of the epithelium and enveloped by supporting cells. In addition, from the beginning of day 2 PH, we observed cells with highly similar characteristics to those of CCs (absence of knob, abundant mitochondria and filamentous material in apical cytoplasm, numerous microtubules, and envelopment by supporting cells) but with cilia still remaining on their noninvaginated apical surface. We conclude that these cells may correspond to immature CCs in which the crypt, the final feature of their morphological differentiation, has not yet formed.
ISSN:0379-864X
1464-3553
DOI:10.1093/chemse/bjp091