The cytotoxic effects of resin-based sealers on dental pulp stem cells

• Published in: International endodontic journal 2010-08, Vol.43 (8), p.646-653
• Main Authors: Trubiani, O., Caputi, S., Di Iorio, D., D'Amario, M., Paludi, M., Giancola, R., Di Nardo Di Maio, F., De Angelis, F., D'Arcangelo, C.
• Format: Article
• Language: English
• Subjects: Adhesives; Adolescent; bonding systems; CD105 antigen; CD29 antigen; CD44 antigen; CD73 antigen; CD90 antigen; Cell adhesion; Cell Differentiation; Cell Survival - drug effects; Cells, Cultured; Cytotoxicity; Data processing; Dental pulp; Dental Pulp - cytology; Dental Pulp - drug effects; Dentin-Bonding Agents - toxicity; Dentistry; Flow Cytometry; Humans; Immunophenotyping; mesenchymal stem cells; Mesenchymal Stromal Cells - drug effects; Mesenchyme; Optical density; Resin Cements - toxicity; Statistical analysis; Stem cells
• ISSN: 0143-2885; 1365-2591
• DOI: 10.1111/j.1365-2591.2010.01720.x

Trubiani O, Caputi S, Di Iorio D, D’Amario M, Paludi M, Giancola R, Di Nardo Di Maio F, De Angelis F, D’Arcangelo C. The cytotoxic effects of resin‐based sealers on dental pulp stem cells. International Endodontic Journal. Aim  To evaluate the effect of four current resin‐based adhesives on expanded ex vivo human dental pulp mesenchymal stem cells (DP‐MSCs). Methodology  Dental pulp mesenchymal stem cells were derived from dental pulps of ten donors. After in vitro isolation, dental pulp stem cells were analysed using flow cytometry. The immunophenotype of DP‐MSCs disclosed the homogeneous expression of the mesenchymal‐related antigens CD29, CD44, CD73, CD90, CD105, CD166. DP‐MSCs were exposed to four different commercially available bonding systems (CMF Bond, Prime&Bond NT, Clearfil S3 Bond, XP Bond), and after 24, 48 and 72 h of incubation the morphological features and the cell growth were analysed. Moreover, the cell viability was evaluated at the same times by MTT assay. Data were statistically analysed using a two‐way anova and Holm–Sidak method (α set at 0.05). Results  Significant differences were observed between the four groups when comparing DP‐MSCs appearance. DP‐MSCs survived and proliferated without inhibition in the presence of CMF Bond adhesive. On the contrary, microscopic evaluation of the other three groups revealed extensive cytotoxic effects from the dentine bonding agents. The MTT assay revealed no statistically significant differences in cell viability after 72 h between the control group and CMF Bond group. All the other experimental groups had statistically lower optical density values. Conclusions  CMF Bond adhesive allowed human dental pulp stem cells to survive and proliferate. All of the other dentine bonding agents had extensive cytotoxic effects.