Simultaneous Automated determination of Free and Total Sulfisoxazole and Sulfamethoxazole in Plasma and urine

A fully automated method for the determination of sulfisoxazole, N4-acetylsulfisoxazole, sulfamethoxazole, and N4-acetylsul-famethoxazole in human plasma and urine was developed. Untreated plasma is analyzed by automation of dialysis, hydrolysis, color development, and quantitation. The method has a...

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Bibliographic Details
Published in:Journal of pharmaceutical sciences 1979-11, Vol.68 (11), p.1387-1392
Main Authors: Weinfeld, Robert E., Lee, Teh Lo
Format: Article
Language:English
Subjects:
Antibacterial agents-sulfisoxazole
Antibacterial agents—sulfisoxazole, sulfamethoxazole, automated analysis, human plasma and urine
Autoanalysis
automated analysis
Automated analysis-sulfisoxazole and sulfamethoxazole
Automated analysis—sulfisoxazole and sulfamethoxazole, human plasma and urine
Dialysis
human plasma and urine
Humans
Hydrolysis
Methods
sulfamethoxazole
Sulfamethoxazole - analysis
Sulfamethoxazole - blood
Sulfamethoxazole - urine
Sulfamethoxazole-automated analysis
Sulfamethoxazole—automated analysis, human plasma and urine, with sulfisoxazole
Sulfisoxazole - analysis
Sulfisoxazole - blood
Sulfisoxazole - urine
Sulfisoxazole-automated analysis
Sulfisoxazole—automated analysis, human plasma and urine, with sulfamethoxazole
with sulfamethoxazole
with sulfisoxazole
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Description
Summary:A fully automated method for the determination of sulfisoxazole, N4-acetylsulfisoxazole, sulfamethoxazole, and N4-acetylsul-famethoxazole in human plasma and urine was developed. Untreated plasma is analyzed by automation of dialysis, hydrolysis, color development, and quantitation. The method has a sensitivity limit of 2 μg/ml of plasma and has been used successfully to determine sulfonamide levels following administration of sulfisoxazole and a combination drug product containing sulfamethoxazole and trimethoprim in humans. Samples are processed at the rate of 40 per hour, with a minimum of sample handling, data reduction, and materials.
ISSN:0022-3549
1520-6017
DOI:10.1002/jps.2600681113