Human plasma angiotensinogen: a review of purification procedures

The current status of the purification and characterization of human angiotensinogen is reviewed. One problem encountered in the past has been the copurification of a protein with similar porperties. This protein has tentatively been designated alanine-protein. An efficient separation of angiotensin...

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Bibliographic Details
Published in:Molecular and cellular biochemistry 1979-09, Vol.27 (1), p.47-56
Main Authors: Tewksbury, D A, Dart, R A
Format: Article
Language:English
Subjects:
Amino Acids - analysis
Angiotensinogen - blood
Angiotensinogen - isolation & purification
Angiotensins - isolation & purification
Blood Proteins - isolation & purification
Carbohydrates - analysis
Chromatography - methods
Electrophoresis, Polyacrylamide Gel
Humans
Isoelectric Focusing
Renin - blood
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Summary:The current status of the purification and characterization of human angiotensinogen is reviewed. One problem encountered in the past has been the copurification of a protein with similar porperties. This protein has tentatively been designated alanine-protein. An efficient separation of angiotensinogen and alanine-protein was obtained on a zinc chelate column. Alanine-protein has been purified and its amino acid and carbohydrate composition determined. The COOH-terminal amino acid and the NH2-terminal amino acid were determined to be serine and alanine, respectively. Alanine-protein exhibited multiple forms on isoelectric focusing.
ISSN:0300-8177
1573-4919
DOI:10.1007/BF00849278