Sensitive High-pressure Liquid Chromatographic Determination of Disopyramide and Mono-N-dealkyldisopyramide

A high-pressure liquid chromatographic procedure for the accurate determination of disopyramide and its chief metabolite in plasma is presented. The method is suitable for monitoring patients receiving disopyramide therapy. A reversed-phase cyanopropylsilane column is utilized with a mobile phase of...

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Bibliographic Details
Published in:Journal of pharmaceutical sciences 1979-10, Vol.68 (10), p.1318-1320
Main Authors: Nygard, Gloria, Shelver, William H., Khalil, S.K.Wahba
Format: Article
Language:English
Subjects:
analysis
Antiarrhythmic agents-disopyramide
Antiarrhythmic agents—disopyramide, high-pressure liquid chromatographic analysis, plasma, humans
Chromatography, High Pressure Liquid
Dealkylation
Disopyramide - blood
Disopyramide-analysis
Disopyramide-metabolites
Disopyramide—analysis, high-pressure liquid chromatography, plasma, metabolites, humans
Disopyramide—metabolites, analysis, high-pressure liquid chromatography, humans
high-pressure liquid chromatographic analysis
high-pressure liquid chromatography
Humans
metabolites
Methods
Mono-N-dealkyldisopyramide-analysis
Mono-N-dealkyldisopyramide—analysis, high-pressure liquid chromatography, plasma, humans
plasma
Pyridines - blood
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Description
Summary:A high-pressure liquid chromatographic procedure for the accurate determination of disopyramide and its chief metabolite in plasma is presented. The method is suitable for monitoring patients receiving disopyramide therapy. A reversed-phase cyanopropylsilane column is utilized with a mobile phase of 50% acetonitrile and 50% 0.01 M sodium acetate buffer at pH 4.0. Absorption was monitored at 254nm with a detection limit of 0.2 μg/ml of plasma. The reproducibility and precision of the procedure were demonstrated on samples containing 0.50-12 μg/ml of plasma.
ISSN:0022-3549
1520-6017
DOI:10.1002/jps.2600681033