Cytochemistry and freeze-fracture of membranes isolated from the electrocyte of Electrophorus electricus (L.)

Membranes were isolated from the main electric organ of Electrophorus electricus and studied by means of cytochemistry and freeze-fracture. The membrane fractions consisted of vesicles inside-in as determined by localization of anionic sites using colloidal iron and cationized ferritin particles. Th...

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Bibliographic Details
Published in:Cell and tissue research 1979-11, Vol.202 (2), p.275-281
Main Authors: de Souza, W, Benchimol, M, Somlo, C, Machado, R D, Hasson-Voloch, A
Format: Article
Language:English
Subjects:
Animals
Anions
Binding Sites
Cell Membrane - metabolism
Cell Membrane - ultrastructure
Electric Organ - metabolism
Electric Organ - ultrastructure
Electrophorus - metabolism
Freeze Fracturing
Histocytochemistry
Microscopy, Electron
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Summary:Membranes were isolated from the main electric organ of Electrophorus electricus and studied by means of cytochemistry and freeze-fracture. The membrane fractions consisted of vesicles inside-in as determined by localization of anionic sites using colloidal iron and cationized ferritin particles. The anionic sites were not homogeneously distributed on the surface of the vesicle. Freeze-fracture showed the presence of intramembranous particles associated with either protoplasmic (P) or extracellular (E) faces of the membrane. Regions of the membrane without particles were observed. The results are discussed in relation to the existence of association between intramembranous particles and membrane receptors.
ISSN:0302-766X
1432-0878
DOI:10.1007/BF00232241