Mason-Pfizer Monkey Virus Isolated From Spontaneous Mammary Carcinoma of a Female Monkey. I. Detection of Virus Antigens by Immunodiffusion, Immunofluorescent, and Virus Agglutination Techniques

Antisera to Mason-Pfizer monkey (M-PM) virus prepared in rabbits and monkeys reacted specifically with M-PM virus-associated antigens in microimmunodiffusion tests producing five precipitin lines. Two of the five antigens, “d” and “e,” were released after degradation of virion with ether, freezing a...

Full description

Saved in:
Bibliographic Details
Published in:JNCI : Journal of the National Cancer Institute 1971-06, Vol.46 (6), p.1325-1334
Main Authors: Ahmed, Mumtaz, Mayyasi, Sami A., Chopra, Harish C., Zelljadt, Inna, Jensen, Erlins M.
Format: Article
Language:English
Subjects:
Agglutination Tests
Animals
Antigens - analysis
Antigens, Viral - analysis
Cell Line
Culture Techniques
Embryo, Mammalian - immunology
Embryo, Nonmammalian
Fluorescent Antibody Technique
Immune Sera
Immunodiffusion
Immunoglobulins - isolation & purification
Lymphocytes - immunology
Mammary Neoplasms, Experimental - microbiology
Viruses, Unclassified - immunology
Viruses, Unclassified - isolation & purification
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Antisera to Mason-Pfizer monkey (M-PM) virus prepared in rabbits and monkeys reacted specifically with M-PM virus-associated antigens in microimmunodiffusion tests producing five precipitin lines. Two of the five antigens, “d” and “e,” were released after degradation of virion with ether, freezing and thawing, or disruption by sonic vibration. These antigens diffused readily in the gel matrix, did not sediment at 92,000 × g for 7 hours, and were also present in the M-PM virus-infected monkey and human NC-37 cell cultures. Antiserum to monkey cell-derived M-PM virus caused specific agglutination of M-PM virions recovered from monkey or human cells, indicating that viral coat antigens from these two sources are similar or identical. Fluorescent conjugates, prepared with concentrated immunoglobulin, specifically stained the infected cells, with fluorescence limited to the cytoplasm.
ISSN:0027-8874
1460-2105
DOI:10.1093/jnci/46.6.1325