High-Resolution Studies of Uniformly ¹³C,¹⁵N-Labeled RNA by Solid-State NMR Spectroscopy

Chilling out: Solid‐state 13C NMR correlation spectroscopy was used to assign the signals of a uniformly labeled RNA hairpin in frozen aqueous solution. Conformational analysis shows that solutions of biologically relevant RNAs can freeze without significant changes in RNA structure and without crit...

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Bibliographic Details
Published in:Angewandte Chemie (International ed.) 2010-06, Vol.49 (28), p.4747-4750
Main Authors: Cherepanov, Alexey V, Glaubitz, Clemens, Schwalbe, Harald
Format: Article
Language:English
Subjects:
Base Sequence
conformation analysis
freeze-quenching
Models, Molecular
NMR spectroscopy
Nuclear Magnetic Resonance, Biomolecular
Nucleic Acid Conformation
RNA
RNA - chemistry
solid-state structures
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Summary:Chilling out: Solid‐state 13C NMR correlation spectroscopy was used to assign the signals of a uniformly labeled RNA hairpin in frozen aqueous solution. Conformational analysis shows that solutions of biologically relevant RNAs can freeze without significant changes in RNA structure and without critical loss of resolution and sensitivity in NMR experiments.
ISSN:1433-7851
1521-3773
DOI:10.1002/anie.200906885