High-performance liquid chromatographic method for identification and quantification of three potent liver protective coumarinolignoids-cleomiscosin A, cleomiscosin B and cleomiscosin C-in extracts of Cleome viscosa

• Published in: Biomedical chromatography 2010-09, Vol.24 (9), p.1000-1005
• Main Authors: Kaur, Ranjeet, Kumar, Satyanshu, Chatterjee, Arnab, Chattopadhyay, Sunil K
• Format: Article
• Language: English
• Subjects: Chromatography, High Pressure Liquid - methods; Cleome - chemistry; Cleome viscosa; Cleomiscosin A; Cleomiscosin B; Cleomiscosin C; Coumarins - analysis; HPLC analysis; Molecular Structure; Reproducibility of Results
• ISSN: 0269-3879; 1099-0801
• DOI: 10.1002/bmc.1399

A simple, rapid, accurate and reproducible reverse‐phase HPLC method has been developed for simultaneous identification and quantification of three coumarinolignoids, cleomiscosin A (Cliv A), cleomiscosin B (Cliv B) and cleomiscosin C (Cliv C) in different extracts of the seeds of Cleome viscosa using photodiode array detection at 326 nm. Cliv A, B and C were separated on a Waters symmetry C18 column (250 × 4.6 mm, 5 μm) using the solvent system consisting of a mixture of acetonitrile : methanol (1:2 v/v) and water : acetic acid (99.5:0.5 v/v) as a mobile phase in a gradient elution mode. The calibration curves were linear in the concentration ranges 15–200, 10–80 and 15–180 μg/mL for Cliv A, Cliv B and Cliv C, respectively. The limits of detection and quantification for Cliv A, Cliv B and Cliv C were 15 and 20 μg/mL, 10 and 15 μg/mL and 15 and 20 μg/mL, respectively. The intra‐day and inter‐day precisions were 2.08 and 0.93% for Cliv A , 1.22 and 0.39% for Cliv B and 1.29 and 0.23 for Cliv C respectively. The developed HPLC method was used to identify and quantify Cliv A, Cliv B and Cliv C in different extracts of seed of Cleome viscosa. Copyright © 2010 John Wiley & Sons, Ltd.