Epigallocatechin-3-gallate, a Natural Polyphenol, Inhibits Cell Proliferation and Induces Apoptosis in Human Ovarian Cancer Cells

Epigallocatechin-3-gallate (EGCG) is a polyphenol constituent present in green tea previously shown to inhibit cancer growth. However, studies on human ovarian cancer are limited. This study evaluated, the effects of EGCG as a potential anti-proliferative and pro-apoptotic agent in the human ovarian...

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Bibliographic Details
Published in:Anticancer research 2010-07, Vol.30 (7), p.2519-2523
Main Authors: RAO, Sunita D, PAGIDAS, Kelly
Format: Article
Language:English
Subjects:
Anticarcinogenic Agents - pharmacology
Apoptosis - drug effects
Biological and medical sciences
Catechin - analogs & derivatives
Catechin - pharmacology
Cell Cycle - drug effects
Cell Growth Processes - drug effects
Cell Line, Tumor
Cell Survival - drug effects
DNA, Neoplasm - biosynthesis
Dose-Response Relationship, Drug
Female
Female genital diseases
Gynecology. Andrology. Obstetrics
Humans
In Situ Nick-End Labeling
Medical sciences
Ovarian Neoplasms - drug therapy
Ovarian Neoplasms - pathology
Tumors
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Summary:Epigallocatechin-3-gallate (EGCG) is a polyphenol constituent present in green tea previously shown to inhibit cancer growth. However, studies on human ovarian cancer are limited. This study evaluated, the effects of EGCG as a potential anti-proliferative and pro-apoptotic agent in the human ovarian cancer line, SKOV-3. The MTS assay which measures metabolic activity of cells, bromodeoxyuridine (BrdU) incorporation assay, and flow cytometry were used for the cell proliferation studies and cell morphology, DNA fragmentation analysis and the TUNEL assay for apoptotic effects. The EGCG treated SKOV-3 cells showed inhibition of cell viability and proliferation in a dose-dependent manner. Furthermore, EGCG-mediated SKOV-3 cell growth inhibition was associated with apoptotic changes as evident by cell cycle arrest and accumulation of cells in the apoptotic phase, cell morphological changes, DNA fragmentation and TUNEL-positive apoptotic cells. In SKOV-3 cells, EGCG inhibits cell proliferation via DNA synthesis reduction and induces apoptotic cell death via DNA damage, thus elucidating a novel, plausible mechanism of EGCG anti-tumorigenic property.
ISSN:0250-7005
1791-7530