Overexpression of MMP-13 Gene in Colorectal Cancer with Liver Metastasis

• Published in: Anticancer research 2010-07, Vol.30 (7), p.2693-2699
• Main Authors: YAMADA, Takanobu, OSHIMA, Takashi, KUNISAKI, Chikara, SHIOZAWA, Manabu, MORINAGA, Soichiro, AKAIKE, Makoto, RINO, Yasushi, TANAKA, Katsuaki, MASUDA, Munetaka, IMADA, Toshio, YOSHIHARA, Kazue, TAMURA, Shuzo, KANAZAWA, Amane, INAGAKI, Daisuke, YAMAMOTO, Naoto, SATO, Tsutomu, FUJII, Shoichi, NUMATA, Kazushi
• Format: Article
• Language: English
• Subjects: Aged; Biological and medical sciences; Colorectal Neoplasms - enzymology; Colorectal Neoplasms - genetics; Colorectal Neoplasms - pathology; Female; Gastroenterology. Liver. Pancreas. Abdomen; Gene Expression; Humans; Intestinal Mucosa - enzymology; Liver Neoplasms - enzymology; Liver Neoplasms - genetics; Liver Neoplasms - secondary; Lymphatic Metastasis; Male; Matrix Metalloproteinase 13 - biosynthesis; Matrix Metalloproteinase 13 - genetics; Matrix Metalloproteinase 7 - biosynthesis; Matrix Metalloproteinase 7 - genetics; Matrix Metalloproteinase 9 - biosynthesis; Matrix Metalloproteinase 9 - genetics; Medical sciences; Middle Aged; Neoplasm Metastasis; Stomach. Duodenum. Small intestine. Colon. Rectum. Anus; Tissue Inhibitor of Metalloproteinase-1 - biosynthesis; Tissue Inhibitor of Metalloproteinase-1 - genetics; Tumors
• ISSN: 0250-7005; 1791-7530

Matrix metalloproteinase-7 (MMP-7), MMP-9, MMP-13, and tissue inhibitor of matrix metalloproteinase-1 (TIMP-1) are considered to have important roles in the invasiveness and outcomes of colorectal cancer (CRC). This study examined the clinicopathological significance of the relative expression of these genes in patients with colorectal cancer, especially as related to liver metastasis. The study analysed surgical specimens of cancer tissue and adjacent normal mucosa obtained from 202 patients with untreated colorectal cancer. MMP-7, MMP-9, MMP-13, TIMP-1, and beta-actin mRNA of cancer tissue and adjacent normal mucosa were measured by quantitative real-time, reverse-transcriptase polymerase chain reaction. Expression levels of MMP-7, MMP-9, MMP-13 and TIMP-1 were higher in cancer tissue than in adjacent normal mucosa. On analysis of the relations between gene expression and clinicopathological factors, MMP-13 expression was found to correlate with liver metastasis. Moreover, MMP-13 expression levels were higher in tumour tissue with liver metastasis than in that without liver metastasis. It is concluded that MMP-13 gene expression is a useful predictor of liver metastasis in patients with CRC.